Unit 2B assignment of applied science level 3 extended national diploma. achieved overall grade merit in unit with 3 assignments achieving distinctions. Overall course grade D*D*D.
Undertake chromatographic techniques to identify components
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, September 1, 2023
BTEC applied science Level 3
Chromatography
As a technician assistant at chemcalequip I will be writing a report explaining what
chromatography is, different uses, different types and how to calculate Rf values.
Introduction:
Chromatography is a method used to separate chemical mixtures and identify substances.
From this you can find out the purity of a chemical substance. It relies on moving a liquid or
gas over a stationary paper or powder.
There are many types of chromatography, but the top ones are:
SPEAK ABOUT FEW TYPES OF CHROMATOGRAPHY
Chromatography is used in industrial processes to purify chemicals, test for trace amounts
of substances, separate compounds and to test procedures for quality control.
The uses of chromatography in everyday life: (3)
- Drug testing (gas chromatography)
- Food safety testing
- Alcohol testing
- Forensic investigations (paper chromatography & ink chromatography)
- Vaccinations
- Biological research
Rf value:
The retention factor (Rf) is defined as the distance travelled by the compound divided by the
distance travelled by the solvent.
R
f
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di st anc et r av el l ed b y sol ut e
di st ance t r av el l ed b y sol v ent
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