100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Summary notes on Differences in DNA between individuals of the same species can be exploited for identification and diagnosis of heritable conditions – AQA A Level Biology Summary Notes £3.99   Add to cart

Summary

Summary notes on Differences in DNA between individuals of the same species can be exploited for identification and diagnosis of heritable conditions – AQA A Level Biology Summary Notes

 9 views  0 purchase

Summary notes on Differences in DNA between individuals of the same species can be exploited for identification and diagnosis of heritable conditions – AQA A Level Biology Summary Notes A* and A quality AQA mark scheme specific notes

Preview 1 out of 4  pages

  • September 8, 2023
  • 4
  • 2023/2024
  • Summary
All documents for this subject (66)
avatar-seller
studytogether
3.8.4.2 Differences in DNA between individuals of the same species can be exploited for
identification and diagnosis of heritable conditions – AQA A Level Biology Summary Notes

Use of labelled DNA probes and DNA hybridisation to locate specific alleles of genes

DNA probe
- Piece of single stranded DNA with base sequence that is complementary to a specific allele
you wish to identify
- Probe is labelled with radioactive or fluorescent tag

To use DNA probe, DNA sample is heated or placed in alkali
Causes DNA sample to become single stranded
DNA probe is added to main DNA sample
If DNA sample is complementary to DNA sample (ie DNA sample contains allele of interest) then the
probe will complementary base pair and bind to sample – this is DNA hybridisation
Sample then washed to remove unbound probe
DNA is colourless so to determine whether or not the probe has bound to DNA sample, the results
are visualised using laser (fluorescent tag on probe) or autoradiogram (radioactive tag on probe)




Application about DNA probs and DNA hybridisation

Application 1 – Soutern Blot
- DNA/RNA fragments seperated by size via gel electrophoresis
- Then identify if DNA fragments contain given allele
- Cannot directly add DNA probe to gel
- So DNA from gel is imprinted and transferred onto solid support (nitrocellulose or nylon
paper)
- This paper is then unproved

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller studytogether. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for £3.99. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

67096 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy revision notes and other study material for 14 years now

Start selling
£3.99
  • (0)
  Add to cart