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biochemistry - centrifugation
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content: 1) centrifugation of blood 2) centrifugation 3)Revolution per minute (RPM) vs Relative centrifugal field (RCF) 4) Relative centrifugal field 5) Rotor types 6) Types of centrifuges 7)Types of centrifugation 8) Sucrose density gradient centrifugation to isolate polysome 9) Isopy...

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  • October 28, 2023
  • 7
  • 2023/2024
  • Lecture notes
  • Nigel
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Centrifugation

Centrifugation of blood
 A tube of blood will separate if given enough time.
 Centrifugation will separate most particles into fractions in a short amount of time
 The particles’ settling velocity in centrifugation is a function of their:
- Applied force
- Density difference between the particle and the liquid
- Size and shape of the molecule’s centrifugal acceleration
- Viscosity of the medium




Centrifugation
 Separates (or concentrates) particles in a liquid medium based on differences in size,
shape and density.
 Particles will sediment in a liquid of appropriate density if left long enough. Different
particles will sediment at different rates.
 Centrifugation uses centrifugal force to speed the sedimentation process up

, Revolutions per minute (RPM) vs Relative Centrifugal Field (RCF)
 RPM: how fast the centrifuge goes
 RCF: the amount of acceleration or force exerted on the sample in a centrifuge, i.e., a
function of the rotational speed of the rotor and the radius of the rotation.




Relative Centrifugal Field
 RCF is expressed as a multiple of the earth’s gravitational field (g=9.807 ms-2).
 The ratio of the weight of the particle in the centrifugal field to the weight of the
same particle when acted on by gravity alone and is then referred to as the relative
centrifugal field.
Formula: RCF=1.12x R x (speed in rpm/1000)2
R: radius in mm and rpm: revolutions per minute

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