Some examples from this set of practice questions
1.
STR
Answer: Short Tandem Repeat/ microsatellites. 1-6bp long
2.
Fluorescence Detection
Answer: Dye molecule is excited by a laser. The dye emits light which can then be detected.
3.
Labelling DNA
Answer: 3 methods: -Labelling 5\' end of one primer -Incorporate labelled dNTPs into the product -Use a fluorescent intercalating dye
4.
Collection Software Conditions
Answer: Voltage, injection time, injection amount
5.
GeneMapper
Answer: Processes raw data and displays the data used to assign alleles to the DNA profile.
6.
Interpretation of the Peaks
Answer: Interpret artefacts, peak balance, peak morphology and peak height/peak area ratio.
7.
Stutter peaks
Answer: A peak one repeat less than the real allele because of mispairing in the PCR reaction. Affects dinucleotides the most because they\'re shorter. To solve- use complex repeats
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