Testing urine process flashcard. OCR A: A level biology revision notes. Module 5: Communication, Homeostasis and Energy. A* biology student's notes. Summary of key content to be memorised. Process that needs to be memorised from specification.
Testing Urine – Pregnancy Tests: Testing Urine – Pregnancy Tests:
When the embryo implants on the uterine
lining, it produces human chorionic
gonadotropin (hCG).
hCG can be detected from 6 days after
conception.
Urinate on the test stick.
hCG binds to mobile monoclonal
antibodies (which are attached to a blue
bead of dye).
The mobile antibodies move down the test
stick.
There’s a fixed site which monoclonal
antibody-hCG complexes bind to, holding
the beads of dye in place along a line.
This is the test line.
Beyond this point is a fixed site where
antibodies with no hCG bind to.
This is the control line that shows the test
has worked.
Testing Urine – Anabolic Steroid Tests: Testing Urine – Anabolic Steroid Tests:
Tests for steroids look for the substance
itself or a product of its break down.
Gas chromatography & mass spectroscopy
are used to analyse the samples:
The sample is vaporised & passed through
a polymer containing column.
The substances in the polymer are
separated out due to different movement
speeds.
The mass spectrometer converts these
compounds into ions & separates these by
mass & charge.
The results are analysed & compared to
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