Introduction lectures
Animal cell culture
Cell proliferation and death
Introduction to oxygen transfer
Shear and oxygen transfer
Metabolism
Medium design
Bioreactors and Process modes
Vaccine production
Upstream
Introduction lectures
Biopharmaceutical
- partly produced using a cell (yeast, E.coli, animal cell, animal)
- complex molecule
- injectable
- expensive
- used for life threatening disease
Defined Undefined
➢ Chemical structure / composition is ➢ Critical product attributes unknown
known ○ Extensive testing in clinical
➢ Critical product attributes known trials
○ Product failures
○ mechanism of action
➢ Process not understood
○ side effects ○ Process fixed early in
➢ Understanding of the process development
○ predict product quality ○ Process for trials =process
for market
○ Suboptimal process based
on old technology
○ Reject good batches/delay in
batch approval
Both make the biopharmaceutical very
expensive
Most are undefined, mainly due to Post translational modifications (PTMs)
Mainly glycosylation, effects:
- activity
- immunogenicity
- in-vivo half life (clearance from body)
- stability (storage)
Production platforms
➢ Bacteria → small proteins,
peptides, non glycosylated and
easily refolded proteins
➢ Yeast → more complex proteins
with limited glycosylation
➢ Animal cells → complex proteins
with extensive PTMs
,Approval path
Preclinical Cells, Basic safety: dosages
guinea Investigational new drug application
pigs
Phase 2 50-200 Effectiveness: optimal dosage,
patients application scheme etc.
Phase 3 Hundreds Final safety and effectiveness
patients Very expensive
pharmaceuticals are very expensive:
- whole process costs 2 billion euros
- only 1 in 10 passes all the trials, 1 needs to make up for the other failures
fails in phase 3 → disaster for the company
Fast track
Orphan drugs
- smal patient group
- live threatening diseases
- clear therapeutic of public health advantage (e.g. COVID)
Biosimilars
= exact copies of originals that have gone off patent
, Animal cell culture
➢ In vitro studies
○ Basic cell physiology
○ Toxicity
○ Drug candidates
○ Food components
➢ Cell therapy
○ CAR-T cell cancer therapy
➢ Tissue engineering
○ cartilage (kraakbeen) repair
○ cultured meat
➢ Pharmaceutical proteins
○ EPO = hormone that will increase red blood cell production
normally made in kidney, EPO used for patients with kidney failure
➢ Viral vaccines
➢ Gene therapy (lecture 9 molecular virology)
○ gene delivery by AAV virus
➢ Sponge derived pharmaceuticals
○ sponge = animal
○ defense mechanism = production of complex chemical molecules
○ sponge cells in bioreactor for production
Cell line development
Some cells can escape this Hayflick limit like cancer cells → better cell line
- Infinite life span
- Acceptable growth rate
- Low growth factor dependence
- Suspension growth
- aneuploid = having an abnormal number of chromosomes in a haploid set
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