Summarised lecture notes for Vesicle Transport which also cover the topic on Endocytosis and Exocytosis. Described the type of coating for Vesicles Transport. This note is suitable for MCQs exams.
ENDOCYTOSIS AND EXOCYTOSIS
Membrane-bound vesicles transport….
- Cargo in their lumen and membranes from the donor compartment to the
recipient compartment
- Vesicles bud from specialised coated regions of the donor compartment
3 membrane coat proteins:
COP II – coats vesicles which transport proteins from ER to the Golgi
COP I – coats vesicles transport proteins from the Golgi back to the ER
Clathrin – coats vesicles transport proteins between the Golgi, lysosomes and plasma
membrane
2 Functions of coat proteins:
- Target transport from the donor to the recipient compartments
- Trigger budding of the vesicle from the donor membrane
Structure of clathrin
- Made up of 3 large and 3 small polypeptide chains: form a 3-legged triskelion
- 3-legged triskelion forms a basket-like convex framework of pentagons and
hexagons on the cytoplasmic side of the membrane
Clathrin attach to the cytosolic side of the membrane by:
1. Adaptin binds to the transmembrane receptors that bind
to soluble cargo for transport.
2. Clathrin attaches to the adaptin to form a coat on the vesicle.
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