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Lecture notes

Expression systems

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• Review available recombinant DNA and protein technology • Review what vectors are available to carry and express a gene of interest • Discuss what benefits the different expression systems have

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  • February 26, 2024
  • 10
  • 2023/2024
  • Lecture notes
  • Neil keegan
  • Bgm3039 - medical biotechnology

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Lecture 2: Expression systems

Protein Expression systems
Therapeutic Proteins
Learning Aims

 Review available recombinant DNA and protein technology
 Review what vectors are available to carry and express a gene of interest
 Discuss what benefits the different expression systems have

Learning Outcomes

 Be able to consider and evaluate the questions that should be asked when deciding
on a suitable expression system
 Outline the advantages and disadvantages of the following expression systems
1. Prokaryotes (eg., E Coli)
2. Methylotrophic and other yeasts
3. Insects
4. Mammalian cell-cultures
5. Transgenic animal / Transgenic plants

Why protein Expression and purification?
A. Basic scientific applications
B. Therapeutic p roteins



Expression systems

BioPharma industry is dependent on;
 recombinant protein expression
 high levels of expression
 reproducible quality


This will allow;
 production of sufficient protein
 analysis and better understanding of protein products
 research and development of new therapeutics

DNA cloning

, Expression Vector features

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