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High throughput screening (drug discovery)
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  • Singhania University
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  • Drug Discovery and Development

High-throughput screening (HTS) is a drug discovery technique that uses automated equipment to test millions of samples for biological activity. HTS is a standard method in the pharmaceutical industry.

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Document information

  • March 1, 2024
  • 51
  • 2023/2024
  • Lecture notes
  • Ankita
  • Only in bachelor's degree

Subjects

  • high throughput screening
  • drug discovery
  • clinical research
  • automated equipment
  • machine
  • medicinal drugs

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High-throughput
screening

, Commonly used terms in drug discovery
• High throughput screen: an optimized, miniaturized assay format that enables the testing of >
100,000 chemically diverse compounds per day.



• Assay: a test system in which biological activity can be detected


• Hit: a molecule with confirmed concentration-dependent activity in a screen, and known chemical
structure. The output of most screens



• Progressible hit: a representative of a compound series with activity via acceptable mechanism of
action and some limited structure-activity relationship information



• Lead: a compound with potential (as measured by potency, selectivity, physico-chemical properties,
absence of toxicity or novelty) to progress to a full drug development programme

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