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ASCP Exam/173 Questions and Answers/100% Verified
ASCP Exam/173 Questions and Answers/100% Verified
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ASCP Exam/173 Questions andAnswers/100% Verified
Which is required to generate cDNA
A. DNA polymerase
B. RNase H
C. Restriction Enzyme
D. Helicase - -B
-What is the function of Taq Polymerase - -recognize dsDNA
-You have a 50 μL primer stock at 9pM/μl and want dilute the stock to
900nM. How much stock should you add(what is the dilution factor) - -2
-What gene causes hypersensitivity to Irinotecan? - -UGT1A
-Which allele causes hypersensitivity to Carbamazepine? - -HLA-b*1502
-What gives MLPA its specificity - -Stuffer Sequence
-How is copy number determined? - -MLPA
-What is the gene that cause resistance to Vancomycin - -vanA
-Which assay is FISH best used for - -Prader Willi
-RNA polymerase II: where is it and what does it do - -Eukaryotic: It
catalyzes the transcription of DNA to synthesize precursors of mRNA and
most snRNA and microRNA. (transcribes mRNA)
-Hazard that is blue and is level 4 what does it mean - -big health hazard
-Dr calls wanting test results but results have not been signed out by a
medical director what do you do - -don't give out the result until it has been
signed out my the MD
-What trinucleotide repeat is involved in Fragile X? - -CGG
-In Fragile X, what is considered a full mutation expansion? - -200+
-What method is used to detect Fragile X? - -Southern blot (now PCR
/mPCR)
, -Which gene is the largest and has deletions/duplications as the most
common mutation types - -DMD
-Which HCV geneotype(s) has/have the best prognosis - -Genotypes 2 &3
-Positive attributes of bDNA - -Can be used for both DNA and RNA
-What type of primer set is best? - -forward primer should complement DNA
strand and reverse primer should mirror and inversely complement the
forward primer
-What are some things that stabilize RNA? - -Inactivated and minimizing
exposure to environmental/endogenous RNases
mRNA: 5'cap
-What is the concentration of DNA with an OD of 2.0 - -100 μg/mL
-Given the following data what can quantifiably be said about DNA when 260
= 1.4 and 280 = 1.1 - -There is 70ng of pure DNA
Sample is contaminated with proteins and phenols
-What is the equation for PCR efficiency - -(1+Σ)^n
-You have a gel where the positive control worked, the negative control
worked, patient 1 had a double band, patient 2 was positive, and patient 3
had no bands. What would you do? - -repeat patient 3
-How do you detect for methylation - -Bisulfate sequencing
-MSI (Micro-Satellite Instability) tests for what? - -Mismatch Repair Genes
-Translocation at t(15, 17) - -Translation at t(15, 17)
-Coumadin can be affect by a mutation in what gene - -CYP2C19-KVORC1
-What virus interfere with kidney transplant patients - -BK
-You have 2 HIV viral load tests 5 days apart 1 being high on day one and 1
being low by day 5. What do you do? - -Look into storage and transport
-You are given a strand of primers. Calculate the Tm - -2˚(AT) + 4˚(GC)
-When doing a multiplex you observe faint bands. What do you do - -Adjust
the annealing temp
, -What do you do when you want to optimize a PCR reaction that generates
short PCR fragments? - -Adjust annealing temperature
-Which is caused by a T-cell rearrangement
- Mantle Cell
- Burkits
- Hodkins Lymphoma
- Sezary - -Sezary
-What protects DNA as it is being replicated - -Single Stranded Binding
Protein
-What opens the DNA double helix up - -Helicase
-What unwinds spiral DNA - -topoisomerase
-What does HCV assays target - -5' UTR region
-How does TaqMan work? - -fluorescence is generated when the quencher is
hydrolyzed
-Which is the best order to limit contamination
- Mastermix/ patient/ positive/ negative
- Patient/ Mastermix/ negative/positive
- Matermix/ negative/ patient/ positive
- Mastermix/ positive/ patient/ negative - -Matermix/ negative/ patient/
positive
-How does Luminex work - -Tests one patient with many beads
-How do histones bind to DNA - -ionic charges
-Histones are associated with what amino acids - -Lysine & Arginine
-Methylation and Histone Modification are examples of what - -epigenetics
-Variations in Maternal & Paternal DNA mean what - -imprinting
-HLA typing over serology has the advantage of detecting what - -null
mutations
-Doctor suspects that a patient has clonality you run a PCR and gel and the
patient sample, the positive control, and the negative control all show a
band. What do you do - -repeat testing
-How do you remove RNases from glassware - -treat with DECP for 4 hours
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