ASCP MLS (Hematology ) EXAM QUESTIONS WITH ACCURATA TE SOLUTIONS . Insufficient centrifugation will result in: A. A false increase in hematocrit (Hct) value B. A false decrease in Hct value C. No effect on Hct value D. All of these options, depending on the patient Verified Answer -A. Insufficient centrifugation does not pack down the red blood cells; therefore, the Hct, which is the volume of packed cells, will increase. Variation in red cell size observed on the peripheral smear is described as: A. Anisocytosis B. Hypochromia C. Poikilocytosis D. Pleocytosis Verified Answer -A. A mature erythrocyte is approximately 7-8 μm in diameter. Variation in normal size is denoted by the term anisocytosis. Hypochromia is a term that indicates increased central pallor in erythrocytes, and poikilocytosis denotes variation in red cell shape. Which of the following is the preferable site for bone marrow aspiration and biopsy in an adult? A. Iliac crest B. Sternum C. Tibia D. Spinous processes of a vertebra Verified Answer -A. The iliac crest is the most frequently used site for bone marrow aspiration and biopsy. This site is the safest and most easily accessible, with the bone just beneath the skin, and neither blood vessels nor nerves are in the vicinity. Mean cell volume (MCV) is calculated using the following formula: A. (Hgb / RBC) × 10 B. (Hct / RBC) × 10 C. (Hct / Hgb) × 100 D. (Hgb / RBC) × 100 Verified Answer -B. MCV is the average "volume" of the red cells. This is obtained by dividing the Hct or packed cell volume (PCV) by the red blood cell (RBC) count in millions per microliter of blood and multiplying by 10. The MCV is expressed in cubic microns ( μm3) or femtoliters (fL). What term describes the change in shape of erythrocytes seen on a Wright's -stained peripheral blood smear? A. Poikilocytosis B. Anisocytosis C. Hypochromia D. Polychromasia Verified Answer -A. Variation in shape of the erythrocytes on a peripheral blood smear is poikilocytosis. Anisocytosis refers to a change in size. Hypochromia is an increase in central pallor in erythrocytes. Polychromasia describes the bluish tinge of the immature erythrocytes (reticulocytes) circulating in the peripheral blood. Calculate the mean cell hemoglobin concentration (MCHC) using the following values: Hgb: 15 g/dL (150 g/L) Hct: 47 mL/dL (0.47) RBC: 4.50 × 106/ μL (4.50 × 1012/L) A. 9.5% (.095) B. 10.4% (.104) C. 31.9% (.319) D. 33.3% (.333) Verified Answer -C. MCHC is the average concentration of Hgb in red cells expressed as a percentage. It expresses the ratio of the weight of Hgb to the volume of erythrocytes and is calculated by dividing Hgb by the Hct, and then multiplying by 100. A decreased MCHC indicates that cells are hypochromic. In this example, (1 5 ÷ 47) × 100 = 31.9%. The reference range for MCHC is 32% -36%. A manual white blood cell (WBC) count was performed. A total of 36 cells were counted in all 9 -mm^2 squares of a Neubauer -ruled hemacytometer. A 1:10 dilution was used. What is the WBC count? A. 0.4 × 10^9/L B. 2.5 × 10^9/L C. 4.0 × 10^9/L D. 8.0 × 10^9/L Verified Answer -A. The formula used for calculating manual cell counts using a hemacytometer is: Number of cells counted × dilution factor × depth factor (10) divided by the area. In this example, 36 × 10 × 10 = 3600 ÷ 9 = 400/mm^3 or 0.4 × 10^9/L. When an erythrocyte containing iron granules is stained with Prussian blue, the cell is called a: A. Spherocyte
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