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MCB 2010 Lab Practical
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- NUR 240 EXAM 2 Practice Questions.
MCB 2010 Lab Practical
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MCB 2010 Lab Practicalacid fast - ANS-__ __ staining, also known as the Ziehl Neelsen stain, is used to identify
specialized bacteria that have waxy mycolic acid in their cell wall. The presence of
mycolic acid in bacteria is rare, only found in two genera: Mycobacteria and Nocardia.
The bacteria that possess mycolic acid are considered "acid fast," whereas the vast
majority of bacteria, those that do not have cell walls containing mycolic acid, are
considered "non-acid fast."
Acid fast stain - ANS-This procedure is commonly used to diagnosis active tuberculosis.
Mycobacterium is acid fast. AFB slides are used to compare cells present in sputum to
known smears of acid fast rods and non-acid fast rods.
Aerobes - ANS-Growth dependent upon presence of free oxygen
Amoeba - ANS-
Anaerobes - ANS-Grow in absence of oxygen
Antiseptic - ANS-A chemical used on living tissue (betadyne or rubbing alcohol)
Bacillus - ANS-Morphology
bacterial lawn - ANS-
bacterial lawn - ANS-A term used by microbiologists to describe the appearance of
bacterial colonies when all the individual colonies on a petri-dish agar plate merge to
form a field or mat of bacteria
Bright field - ANS-The specimen appears darker on a bright background
candidiasis, Candida albicans - ANS-Yeast infection, known as ___, is caused by ___
___
Capsules - ANS-
Capsules - ANS-__ help a bacteria stick to the host tissues and helps a bacterium
evade the body's phagocytic defense mechanism.
, carbol fuchsin, acid alcohol, brilliant green - ANS-During an acid stain: 1) __ __ 2) __ __
(decolorizer) 3) __ __ (counter stain)
Coccobacillus - ANS-Morphology
Coccus - ANS-Morphology
crystal violet, gram's iodine, ethanol, safranin - ANS-During a gram stain: 1) __ __ 2) __
__ (mordant) 3) __ (decolorizer) 4) __ (counter stain)
Cyanobacteria - ANS-Blue/green algae that can be found in almost every terrestrial and
aquatic habitat.
Dark field - ANS-Shows specimen bright on a dark background
Dark field - ANS-What kind of microscopy is this?
Differential Gram Stain - ANS-
Diplococci - ANS-
Direct - ANS-__ stains require heat fixing (which may distort shape and size). Use a
basic dye, such as: methylene blue, brilliant green, crystal violet, saffranin. This
increases contrast between the bacteria and the background.
Direct stain - ANS-
Disinfectant - ANS-Chemical used on inanimate objects to destroy pathogenic microbes
(bleach)
E. coli - ANS-__ is the strongest lactose fermenter
Endospores - ANS-__ resist adverse environmental conditions such as high
temperature and dryness. They are produced by soil dwelling bacteria.
Endospores - ANS-tiny green things
enterobacteriaceae, pseudomonas - ANS-The MacConkey agar is used for detection
and isolation of family ___ and ___.
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