Biotechnology CDE Practice Test
1. What is the name of the technique that allows geneticists to observe DNA fragments
from a DNA sample and compare them based on the lengths of the fragments? -
ANS-Gel Electrophoresis
2. A plant breeder makes a cross between two plants that are both the genotype Aa (Aa
X Aa). How many different genotypes with respect to the A,a genes will be produced in
their offspring? - ANS-3
3. A corn plant has the genotype Bb Rr where BB and Bb plants are resistant to corn
borers while bb plants are not AND RR and Rr plants are resistant to Roundup
Herbicide while rr plants are not. Which of the follow is a genotype that could be made
by selfing this BbRr plant? - ANS-All of the above
4. What are proteins? - ANS-a chain of amino acids
6. (5 on notecards) Which choice is the correct ranking of the following items in order of
smallest to largest?
7. Which is the item below that is true about animal breeding? - ANS-Animal breeders
need to pick the parents carefully
8. What is a difference between mitosis and meiosis? - ANS-meiosis is used to make
gametes that can genetically vary and mitosis makes identical cells
9. For natural sexual reproduction to take place, parents have to be? - ANS-of similar
species
10. Mitosis is used in? - ANS-both of the above
11. A transgenic organism is one that - ANS-has genes inserted into it from another
organism
12. Clones are - ANS-genetically identical organisms
13. Cell division that creates two genetically identical cells is called - ANS-mitosis
, 14. Biotechnology can be used in all of the following EXCEPT - ANS-creating a living
thing entirely from non living molecules
15. The most commonly-used bacterium for gene transfer in plants is -
ANS-Agrobacterium
16. A fertilized egg is known as a - ANS-zygote
17. Why are marker genes often inserted into a cell's DNA along with genes of interest?
- ANS-to visually identify transgenic cells or organisms
18. A masked gene is known as a - ANS-recessive
20. (19 on notecards) Select the statement that is true about animal breeding -
ANS-animal breeders have a goal of improving a trait or traits in their breed or species
of animal
21. Which are reasons why mice can be good model organisms for gene discovery? -
ANS-Mice use a universal genetic code so the DNA sequence of a mouse gene will be
similar to the sequence of the gene that works in a similar way in other animals
22. The reason DNA based detection works to detect a transgenic organism in more
situations than
Protein based detection is.... - ANS-DNA detection works in all tissues of an organism
that is transgenic but protein detection may not
23. Nucleotides are: - ANS-building blocks of genes
24. If the sequence of bases in a strand of DNA is represented by TACCAG, what would
be the
sequence of bases in the complementary DNA strand? - ANS-ATGGTC
25. In mitotic division.... - ANS-new cells are exactly like the original cells
26. Most organisms are _________ meaning that they have two sets of chromosomes,
one from each parent. - ANS-diploid
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