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BICH 411 Exam 2.
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BICH 411 Exam 2True or False: the citric acid cycle is an example of an amphibolic pathway - ANS-true
Lipoamide - ANS-
Lipoamide is a cofactor of which enzyme - ANS-alpha-ketoglutarate dehydrogenase
Which one of the following enzymes of the citric acid cycle catalyzes an irreversible step
- ANS-isocitrate dehydrogenase
citrate synthase
Which of the following conditions decreases the rate of oxidation of acetyl CoA by the
citric acid cycle - ANS-a low NAD+/NADH ratio
True or False: in eukaryotes the enzymes of the citric acid cycle are found in the cytosol
- ANS-false
True of False: the citric acid cycle is an anaerobic pathway - ANS-false
True or False: the only reaction in the citric acid cycle that produces a carbon-carbon
bond is catalyzed by citrate synthase - ANS-true
Which enzyme is unique to glyoxylate cylce - ANS-isocitrate lyase
malate synthase
The only reaction of the citric acid cycle that provides substrate level phosphorylation is
catalyzed by - ANS-succinyl CoA synthase
Which of the following is not a property of the E1 enzyme of the pyruvate
dehydrogenase complex - ANS-Binds NAD+ at its active site
Which of the following enzyme mechanisms involves a phosphorylated histidine
intermediate - ANS-succinyl CoA synthetase
Order the coenzymes according to their involvement in the pyruvate dehydrogenase
complex - ANS-TPP, Lipoamide, CoA-SH, FAD, and NAD+
FAD - ANS-
,FAD is a cofactor of which enzyme - ANS-succinate dehydrogenase
In mammalian tissues, isocitrate dehydrogenase is allosterically stimulated by - ANS-a
high NAD+/NADH ratio
Which of the following enzyme activities would not be decreased by a thiamine
deficiency - ANS-pyruvate carboxylase
Fluoroacetate inhibits the citric acid cycle. Fluoroacette is metabolized into a product
that inhibits - ANS-aconitase
Which of the following enzymes catalyzes an oxidative decarboxylation reaction -
ANS-isocitrate dehydrogenase
True or false: the activated form of eukaryotic pyruvate dehydrogenase complex is the
phosphorylated form - ANS-false
Which of the following enzymes catalyzes an anaplerotic reaction - ANS-pyruvate
carboxylase
The conversion of citrate into isocitrate by aconitase involves - ANS-a dehydration
followed by hydration
What are the three enzymes that compose the pyruvate dehydrogenase complex -
ANS-E1, E2, and E3
What is E1 - ANS-pyruvate dehydrogenase
What is E2 - ANS-dihydrolipoyl transacetylase
What is E3 - ANS-dihydrolopoyl dehydrogenase
What is the enzyme and coenzymes of the oxidation of dihydrolipoamide - ANS-E3,
FAD, lipoamide, and NAD
What is the enzyme and coenzymes of the oxidation of FADH2 - ANS-E3, FAD, and
NAD
What is the enzyme and coenzymes of the liberation of CO2 - ANS-E1 and TPP
, What is the enzyme and coenzymes of the formation of acetyl CoA - ANS-E2,
lipoamide, and CoA
What is the enzyme and coenzymes of the formation of acetyllipoamide - ANS-E1, TPP,
lipoamide, and CoA
What is the function of the citric acid cycle - ANS-harvest high energy electrons from
carbon fuels
central metabolic hub of the cell, the gateway of aerobic metabolism
What does the citric acid cycle produce - ANS-intermediates that are precursors for fatty
acids, amino acids, nucleotide bases, cholesterol, and porphoryins
Where does the citric acid cycle occur - ANS-mitochondria
Which enzymes carry out oxidative decarboxylation - ANS-isocitrate
alpha ketoglutarate dehydrogenase
Which enzyme carries our substrate level phosphorylation - ANS-succinyl CoA
synthetase
Which enzymes carry out hydration - ANS-aconitase
fumarase
Which enzyme carry out condensation - ANS-citrate synthase
Which enzymes carry out dehydrogenation - ANS-succinate dehydrogenase
malate dehydrogenase
What is the protein called that transports pyruvate through the inner mitochondrial
membrane into the matrix of the mitochondrian - ANS-pyruvate translocase
Which enzyme carries out the reaction of converting pyruvate into acetyl CoA and CO2
- ANS-pyruvate dehydrogenase
Which enzyme is a multienzyme complex held together by noncovalent interactions -
ANS-pyruvate dehydrogenase
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