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Microbiology Ivy Tech BIOL201.

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Microbiology Ivy Tech BIOL201.

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  • June 22, 2024
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Microbiology Ivy Tech BIOL201
An internal segment of bacterial mRNA sequence consisting of 333 nucleotides will
code for ____________ amino acids. - ANS-111

A mutant strain of an organism having a nutritional requirement for growth is called a(n)
________. - ANS-auxotroph

Anticodons are part of the ____________ molecule. - ANS-transfer RNA

A phenotype is the _____. - ANS-physical appearance of an organism

During ________, the nucleotides of the genetic code are deciphered and converted
into the amino acids that will form a protein. - ANS-translation

For DNA replication, initiation proteins bind to the DNA at the ____________. -
ANS-replication fork

________ is the science of heredity and the field that studies the structure and
expression of the deoxyribonucleic acid (DNA) molecule. - ANS-Genetics

________ is the sequence of bases in the DNA or codons in the RNA that specify a
specific polypeptide. - ANS-Genetic code

________ is when the DNA unwinds and the strands separate. - ANS-Initiation

Pathogenicity islands are: - ANS-gene sequences that code for toxins and other factors.

Plasmids are _____. - ANS-accessory genetic information

Spontaneous mutations could arise from _____. - ANS-atmospheric radiation,
addition of insertion sequences,
DNA replication errors

The Ames test is used to _____. - ANS-identify potential human carcinogens

The antibiotic tetracycline binds to the 30S ribosomal subunit; this prevents: -
ANS-chain initiation

,The complete set of genetic information for an organism or virus is called the ________.
- ANS-genome

The discontinuous synthesis on the lagging strand results from the DNA polymerase
moving away from the replication fork. This results in the formation of short DNA
fragments, called ________, which are eventually joined by a DNA ligase. -
ANS-Okazaki fragments

The DNA replication is carried out: - ANS-by a multiprotein complex of 20 to 30 proteins.

The information in a chromosome may be altered through a permanent change in the
DNA, which is called a ________. - ANS-mutation

Which of the following is not part of an operon? - ANS-Sequencer

Which agent listed below facilitates DNA supercoiling? - ANS-nucleoid-associated
proteins

Which of the following types of RNA function to bring the amino acids to the ribosome
for assembly into a polypeptide chain? - ANS-Transfer RNA

Which one of the following does NOT apply to plasmids? - ANS-They are essential for
growth.

Which one of the following statements is NOT true of the bacterial chromosome? -
ANS-It is diploid.

Which one of the following could NOT cause a change in the mRNA reading frame? -
ANS-Base-pair substitution

Who was able to capture the structure of DNA using X-ray crystallography? -
ANS-Franklin

Select the phrase related most to transformation, conjugation, or transduction. -
ANS-Requires the intervention of a virus - Transduction

Requires contact between donor and recipient cells - Conjugation

Commonly involves F factor transfer - Conjugation

,Was first observed in pneumococci by Griffith - Transformation

Involves an Hfr cell - Conjugation

May follow a release of DNA fragments from a dead cell - Transformation

F factor transfer converts the recipient to F+ - Conjugation

DNA from the environment enters a recipient cell - Transformation

A DNA molecule containing DNA segments spliced together from two or more
organisms is called a ________. - ANS-recombinant DNA molecule

Select the phrase related most to transformation, conjugation, or transduction. -
ANS-Requires the intervention of a virus. - Transduction

Requires contact between donor and recipient cells - Conjugation

Commonly involves F factor transfer - Conjugation

Was first observed in pneumococci by Griffith - Transformation

Involves an Hfr cell. - Conjugation

May follow a release of DNA fragments from a dead cell. - Transformation

F factor transfer converts the recipient to F+ - Conjugation

DNA from the environment enters a recipient cell - Transformation

Involves DNA replication by the rolling circle method - Conjugation

All of the following statements are true EXCEPT: - ANS-plasmids are transferred to
recipient cells by transduction

Which one of the following is NOT an example of genetic recombination? - ANS-Binary
fission

An F- cell is unable to initiate conjugation because it lacks ______. - ANS-an F factor

, When the F factor is transferred to a bacterial cell during conjugation, the recipient cell
__________. - ANS-converts to a donor cell

An Hfr cell ______. - ANS-has a chromosomally integrated F factor

Transformation refers to ______. - ANS-DNA fragments transferred between live donor
and recipient bacterial cells

A(n) _____ seals sticky ends of recombinant DNA segments. - ANS-DNA ligase

The process of conjugation requires that __________. - ANS-two live bacterial cells
come together

_____ are single-stranded DNA molecules that can recognize and bind to a distinctive
nucleotide sequence of a pathogen. - ANS-DNA probes

The F factor in a bacterium is actually a __________. - ANS-plasmid

Besides HGT, __________ is another way to permanently alter the genetic material in a
bacterial cell. - ANS-mutation

The ability of some bacterial genera to undergo transformation depends on their
_________. - ANS-competence

In gram-negative species, the process of conjugation starts with a donor cell that has
a(n) ______. - ANS-F factor

Some strains of a bacterial species contain clusters of up to 25 genes that are
absentfrom other strains of the same species. These are called: - ANS-genomic islands.

In the recombination process called ________, two bacterial cells come together and
the donor cell directly transfers plasmid DNA to the recipient cell. - ANS-conjugation

A chemical agent that disrupts minor chemical reactions and slows microbial
metabolism, which results in a longer time between generations is called a(n) _____. -
ANS-microbiostatic

A halogen often used as a tincture for wound disinfection. - ANS-Iodine

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