Working with the Laboratory Mouse AALAS All Answers Correct
1. What is a common problem with mice housed on wire mesh flooring? Foot injuries can occur due to
the lack of traction and sharp edges on the mesh.
2. What is the recommended method for administering fluid into the stomach of a mous...
Working with the Laboratory Mouse AALAS All Answers Correct
1. What is a common problem with mice housed on wire mesh flooring? Foot injuries can occur due to
the lack of traction and sharp edges on the mesh.
2. What is the recommended method for administering fluid into the stomach of a mouse? The oral
gavage procedure involves inserting a feeding needle into the mouth and threading it down the
esophagus.
3. Which federal policies and guidelines require humane treatment of laboratory animals? All of the
above, including the Animal Welfare Act, PHS Policy, and Guide for the Care and Use of Laboratory
Animals.
4. What is a potential issue with mice used for monoclonal antibody production? They can be
susceptible to subclinical infections after surgery.
5. What is the recommended way to induce gas anesthesia for a mouse? Using an anesthetic induction
chamber is often the easiest and most effective method.
6. What is the goal of appropriate animal care? To minimize pain and distress where possible.
7. Who is responsible for ensuring humane treatment of experimental animals? All individuals involved
in animal research, including researchers, technicians, and veterinarians.
8. What is a recommended method for identifying mice? Ear punching and notching can be used to
permanently identify rodents, although it's not always necessary.
9. What is good practice when performing oral gavage on mice? The needle tip should be inserted
behind the incisors, and the head should be in vertical alignment with the esophagus during the
procedure.
10. When can accreditation by AAALAC International provide an exemption from some mandates of the
Guide for the Care and Use of Laboratory Animals? Never, as accreditation does not exempt institutions
from following the guidelines.
, 11. What is an injectable anesthetic agent commonly used in mice? Pentobarbital is often used as an
injectable anesthetic agent.
12. At what age do mammaries become visible on female mice? Around 9 days of age.
13. Which type of bedding is recommended for postoperative housing for mice? Paper bedding material
can help keep the animals clean and comfortable.
14. What is a common use of injectable anesthesia in mice? It can be used for procedures such as IP
injections, blood collection, or surgery.
15. How deep should a needle be inserted for IP injection in mice? Typically, it's recommended to insert
the needle 0.5 cm deep.
What is the age range when mice are weaned? ✔️21-28 days
While restraining a mouse, a technician should check for which of the following? ✔️All of the Above
Which measure can correct respiratory depression in anesthetized mice? ✔️All of the Above
Which of the following represents an appropriate technique for subcutaneous injections in mice?
✔️Lift some skin on the back of the mouse, and insert the needle into the base of the tent.
How are Salmonella spp. typically transmitted from animals to humans? ✔️By the fecal-oral route
When picking up a mouse from a filter top cage, putting the wire bar lid sideways on the cage is helpful
to: ✔️Reposition your grasp as you take the mouse out of the cage as it will tend to hold on to the wire
bar lid.
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