Southern Blot - CORRECT ANSWER DNA Target
Nucleic acid probe
Purpose: Paternity testing, gene discovery and mapping, mutation analysis
and ID, gene structure
Northern Blot - CORRECT ANSWER RNA Target
Nucleic acid probe
Purpose: Determine transcript structure, processing, and gene expression
,ASCP MB 2024 VERIFIED QUESTIONS AND
ANSWERS
Western Blot - CORRECT ANSWER Protein Target
Protein probe
Purpose: Protein processing, gene expression
Southwestern Blot - CORRECT ANSWER Protein target
DNA probe
Purpose: DNA binding proteins, gene regulation
Eastern blot - CORRECT ANSWER Protein target
Protein probe
Purpose: Modification of Western blot using enzymatic detection (protein
processing, gene expression), detection of specific agriculturally important
proteins
Far-eastern blot - CORRECT ANSWER Lipid target
No probe
Purpose: Transfer separated lipids to membranes for analysis by mass
spectometry
PCR Primers - CORRECT ANSWER Direct DNA synthesis to desired
region
, ASCP MB 2024 VERIFIED QUESTIONS AND
ANSWERS
PCR: dATP, dCTP, dGTP, dTTP - CORRECT ANSWER Building blocks
that extend primers
PCR KCl - CORRECT ANSWER Salt for optimal hybridization of
primers to template
PCR: Tris buffer - CORRECT ANSWER Buffer to maintain optimal pH
for enzyme reaction
PCR: MgCl2 - CORRECT ANSWER Divalent cation, required by enzyme
PCR Polymerase - CORRECT ANSWER Polymerase enzyme that
extends the primers, adds the dNTPs
PCR: copies of template - CORRECT ANSWER Sample DNA that is
being tested
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