Microbiology UTA 2460 - Lab Practical 2
1. Resazurin in Fluid Thioglycolate Medium: Functions as an indicator of oxygen presence in the media.
2. Bacterial Classification Based on Growth Observations: - Obligate Anaerobe: Categorized when growth is only observed in an anaerobe jar, indicating i...
1. Resazurin in Fluid Thioglycolate Medium: Functions as an indicator of oxygen presence in the media.
2. Bacterial Classification Based on Growth Observations:
- Obligate Anaerobe: Categorized when growth is only observed in an anaerobe jar, indicating inability
to grow in the presence of oxygen.
3. Facultative Anaerobe Growth Prediction: Expected to grow throughout the tube in fluid thioglycolate
medium, as they can utilize oxygen when available but also grow in its absence.
4. Pink Color in Fluid Thioglycolate Medium: Indicates the presence of oxygen in the top of the broth.
5. Classification of Human Pathogens and Normal Microbiota: Typically classified as mesophiles,
organisms that thrive at moderate temperatures.
### Environmental Preferences
6. Taq Polymerase Source: The enzyme's optimal activity at 72°C suggests its source organism is likely a
thermophile.
7. Vibrio cholerae pH Preference: Ingestion of antacids lowering the required bacterial load for infection
indicates V. cholerae is likely an alkaliphile, preferring alkaline conditions.
8. Effects of pH on Microbial Metabolism: pH changes can denature enzymes, adversely affecting
microbial metabolism.
9. Storage of Jams and Honey: High sugar content creates hypertonic environments leading to
plasmolysis of bacterial cells, allowing storage at room temperature.
10. Halophiles: Microorganisms capable of growing in salt concentrations as high as 15-30%.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller bestscores1. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for £8.56. You're not tied to anything after your purchase.
