BTC 401 Exam 1 Questions & Answers 2024/2025
Pipettes have a listed volume range, but it is okay to go beyond that, as long as you do not exceed 5% beyond the listed range - ANSWERSFalse
Flow hoods are designed to protect the user, while transfer hoods are designed to protect the sample - A...
Pipettes have a listed volume range, but it is okay to go beyond that, as long as you do not exceed 5%
beyond the listed range - ANSWERSFalse
Flow hoods are designed to protect the user, while transfer hoods are designed to protect the sample -
ANSWERSFalse
When making a solution, if the mass of the solid being added is very small compared to the volume of
the solution, it is the only time we can add the stated volume to solvent rather than diluting to the
volume - ANSWERSTrue
The most common solvent in molecular biology is water, because that is the solvent in the living cell -
ANSWERSTrue
The two major ways of separating DNA from contaminating components is physical separation of
contaminants from the DNA, and chemical degradation of the contaminants - ANSWERSTrue
One way to protect DNA from degradation is to grind the tissue you are extracting while frozen, to keep
degrading enzymes inactivated - ANSWERSTrue
Run to the red means that the positively charged DNA will migrate to the negative or red electrode -
ANSWERSFalse
Fluorometry is extremely accurate way to determine DNA concentration, but it does not give any
information about the purity of DNA - ANSWERSTrue
Restriction enzymes are named for the bacteria genus, species and strain they were isolated from, and
the order in which they are isolated - ANSWERSTrue
, Enzymes with a 4-base recognition sequence are more likely to find their target sequence in a genomic
DNA prep, and thus will produce more and larger DNA fragments - ANSWERSFalse
DNA polymerase links the sugar/phosphate backbone of the DNA strand, and DNA hydrolase forms the
hydrogen bonds between the DNA bases - ANSWERSFalse
Linkers are used to add a primer region or restriction sites to the ends of DNA fragments - ANSWERSTrue
Bacterial cells are limited in the numbers of copies of a given plasmid they can contain, which is normally
around 10 copies per cell - ANSWERSFalse
DNA is readily soluble in water, and thus has a hard time passing through a cell or nuclear membrane -
ANSWERSTrue
Transfer membranes have an overall negative charge, which helps the positively charged DNA to adhere
to the membrane - ANSWERSFalse
Satellite colonies are extremely small and insignificant and will not cause major problems if some of their
cells get included with the cells you want to study - ANSWERSFalse
What are some safety items you should locate before you start working in a lab?
A. fire extinguisher
B. eye wash
C. safety shower
D. all of the above - ANSWERSD. all of the above
What is special about a buffer solution?
A. it maintains its concentration of solvents
B. it maintains its concentration of solutes
C. it maintains temperature
D. it maintains its pH despite addition of acid or basic compounds
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