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BTC 195 Test Questions & Answers 2024/2025
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BTC 195 Test Questions & Answers 2024/2025
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BTC 195 Test Questions & Answers2024/2025
what assay did we use in lab for protein determination - ANSWERSbradford assay
coomassie blue dye - ANSWERSbinds to side chains of specific amino acids
what does the intensity of blue correlate with - ANSWERSconcentration of protein
how is protein concentration measured - ANSWERSby eye or using a spectrophometer
beers law - ANSWERSis solute absorbs light of a particular wavelength, the absorbance is directly
proportional to concentration of solute
equation used to prepare protein standards - ANSWERSM1V1=M2V2
another equation to measure protein standards - ANSWERSC1V1=C2V2
contain aromatic amino acids that absorb UV light at a wavelength of 280nm - ANSWERStyr, trp, phe, his
at what wavelength do proteins have a strong absorbtion at - ANSWERS280nm
Beer's Law equation - ANSWERSA=ecl
A - ANSWERSabsorbance: the readout you get
e - ANSWERSextinction coefficent: how well the molocule absorbs that wavelength
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