Exam (elaborations)
CHEM 212 BIO CHEMISTRY WORK LAB REPORT 1 (pipettes and electrophoresis) GEVENA COLLEGE
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This Experiment document focuses on understanding pipettes and electrophoresis
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lOMoAR cPSD| 42147428CHEM 212 BIO CHEMISTRY
WORK LAB REPORT 1 (pipettes
and electrophoresis) GEVENA
COLLEGE
, lOMoAR cPSD| 42147428
Biochemistry Lab Report
Experiment #: 1
Title: Video and Lab Report
Purpose: The goal of this lab is presented in 2 parts. The first part of the lab is for the
person performing the lab to understand pipettes and how they work and properly
function. The person performing the lab should also be able to distinguish and correctly
use the different types of pipettes in the correct laboratory situation. The final objective
for part 1 goal is for the person recording the data in their lab notebook to properly use
and differentiate if their results are accurate or precise. Part 2 of the goals for this lab is
for the person performing the lab to understand when and why they would need to use
electrophoresis. They would also be able to perform electrophoresis so that it would
help aid in getting the results needed to complete the lab.
Procedure:
Part 1
Types of Glass Pipettes:
• Volumetric
• Graduated
Glass Pipette Procedure:
• Step 1: Put on appropriate lab equipment based on what solutions
and chemicals will be used in the lab that they are performing. Ex:
Gloves, Lab coat, Closed toe shoes, Protective glasses, etc. Also
have the appropriate materials needed for completion of the lab.
• Step 2: Wet thumb and place on the end of the pipette that is not
used in the solution.
• Step 3: Place bulb and extract solution by squeezing bulb until
solution is at measurement line.
• Step 4: Once at the measurement line place pipette of appropriate
beaker and release the solution into the beaker. *If needing to stop
solution from coming out of pipette place thumb over top of pipette.
Automatic Pipette Types
• Differ in size based on how high and low the settings go.
Automatic Pipette Procedure:
• Step 1: Put on appropriate lab equipment based on what solutions
and chemicals will be used in the lab that they are performing. Ex:
Gloves, Lab coat, Closed toe shoes, Protective glasses, etc. Also
have the appropriate materials needed for completion of the lab.
• Step 2: Holding the pipette upright in hand hold down button on the
side of pipette while twisting the knob on top of the pipette to change
value of pipette.
• Step 3: Each automatic pipette has three stops.
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