Gram positive bacteria do not retain CV when gram stained, so they appear pink after counter-staining
with safranin. T/F - False. They do retain crystal violet and appear purple
Complex media is made with precise chemical composition. T/F - False. Complex media has
unknown composition
______ is...
MCB 2610 Lab Practical
Gram positive bacteria do not retain CV when gram stained, so they appear pink after counter-staining
with safranin. T/F - False. They do retain crystal violet and appear purple
Complex media is made with precise chemical composition. T/F - False. Complex media has
unknown composition
______ is an opportunistic pathogen that causes staph infections. - Staphylococcus aureus
A durham tube is included in the LT broth tubes to detect _________, a byproduct of lactose
fermentation - gas production
Brownian motion is the purposeful movement of microorganisms. T/F - False. It is random
vibrations of organisms coming into contact with water molecules
Cells that do not retain crystal violet and appear pink are said to be - Gram negative
Cells that retain crystal violet and appear purple are - Gram positive
Coliforms are a subgroup of enteric bacteria classified by their ability to ferment which sugar? -
Lactose
Define generation time - The amount of time it takes a population to double
Eosin-methylene blue (EMB) agar is used as a confirmed test for coliforms. T/F - True
Fomite examples - cell phone, table, door knob
, Generation time is calculated in log phase. T/F - True
Gram positive bacteria have a thick peptidoglycan layer which retains safranin and appears pink. T/F -
False. Retains crystal violet and appears purple.
Gram stains and spore stains are examples of simple stains. T/F - False. These are differential
staining techniques
Heat staining causes sporulation. T/F - False.
How is casein hydrolysis detected? - Clearing zone around colonies
How many times do you get culture from the tube while performing a T streak? - 1
Is LT broth selective, differential or both? - Both
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