AAB MT MICROBIOLOGY EXAM
QUESTIONS AND ANSWERS
What term describes the morphology of organisms that vary in shape and size? -
Answer-Pleomorphic
Capsules are the basis of which test? - Answer-Serotyping
A mucoid colony on blood agar medium indicates that which of the following
characteristics may be present? - Answer-Organism has a capsule.
What are dark staining granules called? - Answer-Metachromatic
Which of the following organisms are non-motile? - Answer-Shigella and Klebsiella
In the bacterial growth cycle, growth ceases because nutrients are exhausted or toxic
metabolic products have accumulated in which phase? - Answer-Stationary phase
Which of the following genera of bacteria form spores? - Answer-Bacillus and
Clostridium
What term is used to describe bacteria that grow in the absence of atmospheric oxygen
and obtain oxygen from oxygen-containing compounds? - Answer-Anaerobes
What is the temperature at which bacteria grow best known as? - Answer-Optimum
On blood agar plates a small zone of alpha hemolysis surrounded by a zone of beta
hemolysis after refrigeration is known as what type of hemolysis? - Answer-Alpha-prime
hemolysis
What is the most effective method of sterilization? - Answer-Autoclaving
Which of the following is used for quality control for the dry heat oven? - Answer-
Bacillus subtilis
Media that cannot be heated can be sterilized by what alternate method? - Answer-
Filtration
Which one of the following disinfectants is the most effective germicide against bacteria,
spores, tubercle bacilli, fungi, and viruses? - Answer-Chlorine
What is a term that describes a process or treatment that renders a medical device,
instrument, or environmental surface safe to handle? - Answer-Decontamination
What color do gram-positive bacteria stain? - Answer-Purple
, Acid-fast bacteria appear what color microscopically? - Answer-Red
Acid-fast organisms in tissue are best stained by which of the following methods? -
Answer-Kinyoun
Which of the following should be used to remove antimicrobials before culturing? -
Answer-Antimicrobial removal device
Which statement concerning sodium polyanethol sulfonate (SPS) is NOT true? -
Answer-Activates some antimicrobials
What are some functions of sodium polyanethol sulfonate (SPS)? - Answer-Prevents
blood from clotting, prevents phagocytosis, and neutralizes the bactericidal effect of
human serum.
What is the most abundant normal flora in throat cultures? - Answer-Alpha-hemolytic
streptococci
Why is it recommended to stab the blood agar plate when setting up throat cultures? -
Answer-Some streptococci may not produce beta hemolysis under aerobic conditions.
What is the most common pathogen is throat cultures? - Answer-Streptococcus
pyogenes
Nasopharangeal swabs are recommended for the detection of which carriers? - Answer-
H. influenzae, N. meningitidis, and B. pertussis
Why is a first morning urine specimen preferred for urine cultures? - Answer-Specimen
is more concentrated.
What indicates vaginal or urethral contamination of urine? - Answer-Greater than 10
squamous epithelial cells per hpf.
How are smears of cerebrospinal fluid prepared? - Answer-Centrifuged CSF sediment
Smears of CSF are usually stained with which stain? - Answer-Gram stain
Which of the following tests for bacterial antigen on cultures isolated from CSF is more
sensitive and faster? - Answer-Latex agglutination tests
Which of the following is not the cause of a venereal disease? - Answer-Haemophilus
aegyptius
A positive coagulase test is observed for which of the following? - Answer-Clotting of
plasma
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