Microbiology Lab - Exam 2 Questions And All Correct Answers.
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Module
Microbiology lab
Institution
Microbiology Lab
medium - Answer a mixture of nutritional substances on or in which microorganisms grow
autotroph - Answer nutritionally deficient mutants that have lost the ability to synthesize a particular enzyme
many microorganisms in this group:
- can carry out photosynthesis
- usually do not cau...
Microbiology Lab - Exam 2 Questions
And All Correct Answers.
medium - Answer a mixture of nutritional substances on or in which microorganisms grow
autotroph - Answer nutritionally deficient mutants that have lost the ability to synthesize a particular
enzyme
many microorganisms in this group:
- can carry out photosynthesis
- usually do not cause disease
- synthesize organic compounds to obtain energy
heterotroph - Answer an organism that uses compounds to produce biomolecules
autoclave - Answer an instrument for sterilization by means of moist heat under pressure
Autoclaving combines ____ and ____ to destroy high resistant bacteria. - Answer high temp and high
pressure
What are six basic nutritional requirements of every living thing? - Answer Water, carbon, nitrogen,
metallic elements, energy and growth factors such as vitamins
general purpose media - Answer Designed to grow a broad spectrum of microbes that do not have
special growth requirements
enriched media - Answer medium that contains special additives that could promote certain metabolic
activities. (ex. trypticase soy agar, blood agar, yeast extract broth)
- especially used for fastidious bacteria
, selective media - Answer is a nutrient that inhibits the growth of a certain type of microorganisms (ex.
Gram + bacteria) while permitting the growth of a different type (in this example, Gram - )
differential media - Answer medium which will differentiate between bacteria based on how they
change the medium or their appearance on the medium
EMB and HE are both ____ and ____. - Answer selective and differential
EMB and HE are selective for ____ and differential for ____. - Answer selective for gram-negative
bacteria and differential for lactose fermenters.
What do lactose fermenters and nonfermenters look like on each media? - Answer - Organisms which
quickly ferment lactose will give dark-green to black colonies on this medium; these colonies have a
metallic sheen (Ecoli)
- Organisms that are slow fermenters of lactose will give pink to maroon colonies on this medium. Those
organisms that cannot ferment lactose at all give white (colorless) colonies on EMB agar (since
nonfermenters of lactose are often high pathogenic, this is a useful way to identify potential pathogens)
What is Shigella associated with? - Answer a genus associated with bacillary dysentery
HE and EMB are useful for distinguishing between _____. - Answer Enterobacteriaceae
What are Enterobacteriaceae? - Answer a group of short, Gram-negative, facultative anaerobic rods
that are found in the intestinal tract of various mammals, including humans
PEA and MS agar are _____ for ____. - Answer selective for gram-positive bacteria
MS is _____ and _____ for _____. - Answer selective and differential for mannitol fermenters
What bacterial genus is Mannitol Salt (MS) agar used to distinguish? - Answer Staphylococcus -- high
levels of salt in medium are tolerated by its cells
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