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Exam (elaborations)

MIP 300 Study Guide Questions and Answers Test

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  • Module
  • MIP 300
  • Institution
  • MIP 300

MIP 300 Study Guide Questions and Answers Test External function - Answer--Attachment to surfaces -Protection from environment or phage -Horizontal gene transfer -Movement Polysaccharide layers - Answer--Sugars -Capsule -Slime Layer -Glycocalyx Capsule - Answer-well organized layer, ...

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  • September 4, 2024
  • 10
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • MIP 300
  • MIP 300
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MIP 300 Study Guide Questions
and Answers 2024-2025 Test
External function - Answer--Attachment to surfaces
-Protection from environment or phage
-Horizontal gene transfer
-Movement

Polysaccharide layers - Answer--Sugars
-Capsule
-Slime Layer
-Glycocalyx

Capsule - Answer-well organized layer, not easily removed
-Protects against dehydration
-Protects against phagocytosis
-Allows them to adhere.stick

Phagocytosis - Answer-Cell eating
- w/o capule cell compliments bind to cell and mark it for destruction

slime layer - Answer-Less organized, diffuse and easily removed

Glycocalyx - Answer-"Sugar shell"
Encompasses both capsule and slime layer
Have many bacteria in it

Biofilms - Answer-Protected by and attached by layers of polysachharides
-form on teeth roacks or other surfaces
-30% of implants removed because
-Quorum sensing

quorum sensing - Answer-The ability of bacteria to sense the presence of other bacteria
via secreted chemical signals.
once concentration is high enough
-Polysacharide production up
-antibiotic resistance genes goes down
-Flagella down

S-Layer - Answer-Structured proteins ouside cell wall
-Gram -: attaached to LPS
-Gram +: attached PPG

, Cell shape
Protects against pH, osmotic pressure, and antibacterial enzymes
attahcment

Mycolic acid layer - Answer-waxy outer layer in Mycobacterium
Somewhat resistant to:
dehydration
Antibiotics
phagocytosis

TB - Answer--Consumption
-PPD and chest x-rays

Pili - Answer-Small, short hair like appendages (3-10 nm diameter)
-most not for motility
-can twitch for motility
-adhere to host
-proteins

Bright Field Microscope - Answer--produces a dark image against a brighter
background,
-Most common
-Must fix cells
-Color of stain=bacteria color
-100x requires oil
-Light is bet through objective
-Basic dyes binf to neg
-Acidic dyes bind to pos

Dark Field Microscope - Answer--Background is dark, organism is light
-Light is refracted by organism
-in the field, dont have to heat fix or stain
ex.Syphilis

phase contrast microscope - Answer--Shades of grey to show contrast in cell
components
-slight differences in refractive indexes into light intensity
-Motility because no fixing or staining

fluorescence microscopy - Answer--Uses UV light to excite fluorescent molecules
-Some cells take up the dye
-Common in studying viruses

Magnification - Answer-Objective X eyepiece

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