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AAB MT MICROBIOLOGY EXAM WITH COMPLETE QUESTIONS AND CORRECT ANSWERS
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AAB MT MICROBIOLOGY WITH COMPLETE QUESTIONS AND CORRECT ANSWERS
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AAB MT MICROBIOLOGY WITH COMPLETEQUESTIONS AND CORRECT ANSWERS
Bacteriology arrangements: Correct Answer -pairs (diplococci "N.
Gon"; diplobacilli
-chains (streptococci, streptobacilli)
-grape like clusters (staphylococci)
-group of four-tetrads (peptococcus)
-packets of eight-cuboidal (sarcina)
-palisades (corneybacterium)
Capsules are the basis of which test? Correct Answer serotyping
and identification (capsules are antigenic); they are observed
using India ink (negative staining)
representative organisms (Strep pneumo, Kleb pneumo,
Haemophilus influenza, and Neisseria meningitidius)
A mucoid colony on blood agar medium indicates that which of
the following characteristics may be present? Correct Answer
Organism has a capsule.
-it surrounds some bacteria
What are dark staining granules called? Correct Answer
Metachromatic granunles
-affinity for basic dyes
-stains such as Albert, Neisser, and methylene blue
,Which of the following organisms are non-motile? Correct Answer
Shigella and Klebsiella
(with exception to Salmonella--other "ella" are nonmotile)
Shigella (neg) vs Salmonella (pos)
Klebsiella (neg) vs Enterobacter (pos)
Bacillus anthracis (neg) vs most bacillus species (pos) best
observed at 25C
35-37C ,au be inhibitory
In the bacterial growth cycle, growth ceases because nutrients
are exhausted or toxic metabolic products have accumulated in
which phase? Correct Answer Stationary phase
(plateau ceases-nutrients exhausted/toxic)
Which of the following genera of bacteria form spores? Correct
Answer Bacillus and Clostridium
endospores-non feeding, non motile, non reproductive
-highly resistant to heat, drying, freezing; toxic chemical &
radiation.
(Sporulating bacteria-bacteria in which spores are forming)
What term is used to describe bacteria that grow in the absence
of atmospheric oxygen and obtain oxygen from oxygen-containing
compounds? Correct Answer Anaerobes
Aerobes Correct Answer grows in presence of (free) O2
Capnophiles Correct Answer growth enhanced by increased CO2
, aerotolerants Correct Answer does not grow well but tolerate
(survive) in presence of oxygen
What term describes the morphology of organisms that vary in
shape and size? Correct Answer Pleomorphic
i.e. coccobacilli
What is the temperature at which bacteria grow best known as?
Correct Answer Optimum
mesophilic Correct Answer grows best 35-37C; most organisms
will grow at 20C
thermophilic Correct Answer heat loving (50*55C); autoclaving
procedures
psychotrophilic Correct Answer cold loving (10C)-blood bank
contaminancts
On blood agar plates a small zone of alpha hemolysis surrounded
by a zone of beta hemolysis after refrigeration is known as what
type of hemolysis? Correct Answer Alpha-prime hemolysis
-Beta hemolysis (clear, colorless-complete hemolysis)
-alpa hemolysis (green, incomplete hemolysis)
-Gamma hemolysis (no hemolysis)
What is the most effective method of sterilization? Correct Answer
Autoclaving (steam at 121C @ 16 lbs pressure for 15-30 min)-
**QC Bacillus STEAROTHERMOPHILUS
-boiling method (kills all veg-not all spores/viruses (100C for 15-
30 min)
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