CGMBS Exam 1 Questions And Answers With Verified Solutions
9 views 0 purchase
Module
CGMBS
Institution
CGMBS
DNA polymerase delta - Answer-eukaryote: completes replication on the *lagging*
strand; binds more tightly to DNA sliding clamp
nucleotide and base excision repair
Single strand binding proteins - Answer-Bind to the unpaired DNA strands, keeping
them from re-pairing
topoisomerase 1 - Answer-Ma...
CGMBS Exam 1
DNA polymerase delta - Answer-eukaryote: completes replication on the *lagging*
strand; binds more tightly to DNA sliding clamp
nucleotide and base excision repair
Single strand binding proteins - Answer-Bind to the unpaired DNA strands, keeping
them from re-pairing
topoisomerase 1 - Answer-Making single-stranded DNA breaks to relieve supercoiling
at origin
topoisomerase 2 - Answer-An enzyme that breaks both strands (a DNA double helix),
rotates the ends, and seals the break.
*USES ATP*
DNA gyrase - Answer-type of topoisomerase 2
DNA helicase - Answer-An enzyme that unwinds the DNA double helix at origin
Primase - Answer-RNA polymerase: An enzyme that joins RNA nucleotides to make the
primer using the parental DNA strand as a template.
DNA Polymerase I - Answer-A prokaryotic replication enzyme that fills in gaps in the
lagging strand between Okazaki fragments; also proofreads the final strands
DNA replication - Answer-
DNA Polymerase II - Answer-DNA repair
DNA polymerase III - Answer-prokaryotic: In charge of synthesizing nucleotides onto the
leading end in the classic 5' to 3' direction.
Dna polymerase epsilon - Answer-*leading* strand synthesis; requires presence of
helicase
nucleotide and base excision repair
DNA sliding clamp - Answer-keep DNA polymerase firmly attached to the template while
it is synthesizing new strands of DNA
DNA ligase - Answer-an enzyme that eventually joins the sugar-phosphate backbones
of the Okazaki fragments
, template strand or non-coding strand - Answer-The DNA strand that provides the
template for ordering the sequence of nucleotides in an mRNA transcript.
coding strand - Answer-the *strand of DNA* that is not used for transcription and is
identical in sequence to mRNA
Pyrmidines - Answer-cytosine, thymine, uracil
"King TUT lived in a _____"
small nuclear RNA (snRNA) - Answer-Noncoding RNA found in eukaryotes and
involved in splicing, polyadenylation, and other processes in the nucleus.
small interfering RNA (siRNA) - Answer-triggers degradation of other RNA molecules
constitutive transcription - Answer-mRNA transcribed constantly and abundantly
inducible, or regulatory, transcription - Answer-mRNA transcribed only at certain times
during the cell cycle or under particular conditions
When and where does DNA replication take place? - Answer--Synthesis (s phase)
-nucleus
rules of replication - Answer-1. semiconservative
2. starts at "origin"
3. synthesizes in 5-3 direction
4. can be uni or bidirectional
5. semi-discontinuous (leading and lagging strand)
6. RNA primers required
How do we know DNA replication is semiconservative? - Answer-Meselson-Stahl
experiments
15N cells transferred to 14 N cells
origins for prokaryotes vs. eukaryotes - Answer-prokaryotes: single site origin
eukaryotes: multiple sites of origin
what happens if a base mismatch occurs? - Answer-mispaired nucleotide removed by
3-5 proofreading
why does DNA replication only occur in the 5 to 3 direction? - Answer-the incoming
nucleotide undergoes nucleophilic attack by the 3' hydroxyl group of the growing strand
at the alpha phosphorus of the 5' phosphate group.
topoisomerase - Answer-prevents torsion by DNA breaks
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller PatrickKaylian. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for £6.37. You're not tied to anything after your purchase.
