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MCB 3020 - Exam 2 (Chapters 11, 13, 16, 17, 19) Questions and Answers £13.10   Add to cart

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MCB 3020 - Exam 2 (Chapters 11, 13, 16, 17, 19) Questions and Answers

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how is the N-terminus of the polypeptide modified to become a mature protein? - Answer-removal of the N-formyl group from N-fMet ______ are proteins that correct misfolding and suppress incorrect folding as the protein comes out of the E site of rRNA - Answer-chaperones _____ is a chaperone t...

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  • October 9, 2024
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  • MCB 3020
  • MCB 3020
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MCB 3020 - Exam 2 (Chapters 11, 13, 16,
17, 19) Questions and Answers
how is the N-terminus of the polypeptide modified to become a mature protein? -
Answer-removal of the N-formyl group from N-fMet

______ are proteins that correct misfolding and suppress incorrect folding as the protein
comes out of the E site of rRNA - Answer-chaperones

_____ is a chaperone that associates w the ribosome and masks hydrophobic regions
so they don't fold with each other prematurely - Answer-trigger factor

______ is a chaperone that helps iron-sulfur centers obtain their iron atom - Answer-
DnaJ/DnaK/GrpE

_____ is a chaperone that forms a cage for misfolded proteins to be uncoiled and
refolded, away from cytoplasmic crowding - Answer-GroES/GroEL

______ and _____ are chaperones that are cytoplasmic and require ATP - Answer-
DnaJ/DnaK/GrpE and GroES/GroEL

_____ is when proteins are moved from the cytoplasm to/across the plasma membrane
- Answer-translocation

protein ____ refers to the movement of proteins from the cytoplasm to the external
environment - Answer-secretion

the ____ system is the name for the general secretion pathway - Answer-Sec

the ____ -terminal is the signal peptide/sequence - Answer-N

____ (SRP) is a protein-RNA complex that surveys actively translating ribosomes and
will escort the entire ribosome to the membrane. It looks for membrane proteins with
really hydrophobic signal peptides - Answer-signal recognition particle

the alternative route to the membrane is via the _____ protein - Answer-SecA

the _____ system is another translocation route that will only secrete folded proteins -
Answer-Tat

the 3 main translocation systems for gram-positive and gram-negative bacteria are: -
Answer-Sec
SRP
Tat

,after polypeptide translocation, the two final maturation steps are - Answer-1) signal
peptidase removing the signal peptide
2) protein disulfide isomerase (PDI) forming disulfides

_____ is the enzyme that removes the signal peptidee - Answer-signal peptidase

_____ (PDI) is the enzyme that oxidizes cysteine residues to form disulfides - Answer-
protein disulfide isomerase

____ is a second signal that some proteins have at the C-terminus that directs them to
sortase enzymes embedded in the membrane - Answer-sorting signal

_____ is an enzyme that catalyzes the rxn btw the sorting signal and Lipid II, the
peptidoglycan precursor - Answer-sortase

_____ is the peptidoglycan precursor - Answer-Lipid II

protein secretion in gram-______ bacteria requires crossing 2 membranes - Answer-
negative

secreted proteins that cause host injury are called _____ - Answer-effectors

the 3 two-step secretion systems involved in protein secretion in gram-negative cells
are: - Answer-1) type 5
2) type 2
3) type 9

type 2 secretion systems are only found in _____ - Answer-proteobacteria

type 9 secretion systems are only found in ____ - Answer-bacteroidetes

the 5 one-step secretion systems involved in protein secretion in gram-negative cells
are: - Answer-1) type 1
2) type 3
3) type 4
4) type 6
5) type 7

proteins exported by the type ___ secretion system (a one-step system) pass through
an ATP binding cassette, a membrane fusion protein, and a barrel protein - Answer-1

the type ___ secretion system is a one-step system that works as a molecular syringe -
Answer-3

, the type ___ secretion system is a one-step system that is used for DNA transfer, that
works at the cell poles, using integral membrane proteins, ATPases, and a pilus -
Answer-4

the type ____ secretion system is a one-step system that is a contractile weapon used
by bacteria to deliver toxins to neighboring cells, whether bacterial or eukaryotic -
Answer-6

the type ____ secretion system is a one-step system that is found in Mycobacterium
spp. despite that being a gram-positive bacterium. this ss gives important virulence
factors to host cells. it has roles in DNA transfer, cell envelope integrity, sporulation,
metal uptake, and competition for nutrients - Answer-7

________ (OMVs) are made of a lipid bilayer of outer membrane + lipopolysaccharide
and integral outer membrane proteins. the lumen contains molecules found in the
periplasm. these are ONLY FOUND in gram-NEGATIVE bacteria - Answer-outer
membrane vesicles

_____ (EVs) are vesicles that pinch off the plasma membrane and have to cross a thick
peptidoglycan cell wall. these are ONLY FOUND in gram-POSITIVE bacteria - Answer-
extracellular membrane vesicles

_____ initiates the formation of the replication bubble - Answer-DnaA

____ is a protein involved in termination of transcription - Answer-rho

_____ is the subunit/factor on the replisome that recognizes the promoter region -
Answer-sigma factor

the Shine-Dalgarno sequence is found on the _____ - Answer-mRNA

_____ binds to the initiation complex first (translation) - Answer-IF-3

the protein that promotes translation termination is called _____ - Answer-release factor

genes that encode housekeeping functions and are needed almost always are called
_____ - Answer-housekeeping genes

genes that are expressed continuously are called _____ - Answer-constitutive genes

an ______ enzyme is one whose level rises in the presence of a small effector molecule
called an inducer - Answer-inducible

many catabolic enzymes are _____ enzymes - Answer-inducible

many anabolic enzymes are ____ enzymes - Answer-repressible

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