MCB 3020 Chapter 14 - IMVIC Test Exam Questions and Answers
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Module
MCB 3020
Institution
MCB 3020
What does IMViC stand for? - Answer-Indole, Methyl Red, Voges-Proskauer, and Citrate test.
What is the IMViC test used for? - Answer-Used to identify if gut bacteria to ensure water doesn't have pathogens such as salmanella.
Indole test - Answer-Detects the presence of tryptophanase to break ...
MCB 3020 Chapter 14 - IMVIC Test Exam
Questions and Answers
What does IMViC stand for? - Answer-Indole, Methyl Red, Voges-Proskauer, and
Citrate test.
What is the IMViC test used for? - Answer-Used to identify if gut bacteria to ensure
water doesn't have pathogens such as salmanella.
Indole test - Answer-Detects the presence of tryptophanase to break down tryptophan
into indole.
Indole test indicator - Answer-Kovac's reagent
Positive indole test result - Answer-Produces red top layer
Negative indole test result - Answer-Produces colorless/pale orange top layer
Medium used for indole test - Answer-SIM (Sulfide Indole Motility) deep medium =
differential
Methyl red test - Answer-Detects the ability to use glucose for energy and produce
stable acids
Medium used for methyl red test AND Voges-Proskauer test - Answer-MRVP (Methyl
Red Voges Proskaur) Broth. Contains glucose, peptone, and phosphate buffer =
differential.
Positive methyl red test result - Answer-Red broth = acidic
Negative methyl red test result - Answer-Yellow broth = basic
Voges-Proskauer test - Answer-Detects the presence of acetoin.
Voges-Proskauer test indicator - Answer-Barrit's reagent: contains alcoholic α-naphthol
(VP-A) and 40% potassium hydroxide solution (VP-B).
Positive Voges-Proskauer test result - Answer-Produces dark red top layer
Negative Voges-Proskauer test result - Answer-Produces no layer, remains dark yellow
Citrate Utilization Test - Answer-Detects the presence of citrate permease to use citrate
for energy = no glucose used!
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