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Exam (elaborations)

MCB 3020 UF Exam Questions and Answers

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  • Module
  • MCB 3020
  • Institution
  • MCB 3020

Components of the replisome are: - Answer-topoisomerase, single strand binding proteins, DNA polymerase III, helicase Anaerobic respiration - Answer-Uses electron carriers other than oxygen and yields less energy than aerobic respiration Post-transcriptional modifications such as adding a G c...

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  • October 9, 2024
  • 9
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • MCB 3020
  • MCB 3020
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MCB 3020 UF Exam Questions and
Answers
Components of the replisome are: - Answer-topoisomerase, single strand binding
proteins, DNA polymerase III, helicase

Anaerobic respiration - Answer-Uses electron carriers other than oxygen and yields less
energy than aerobic respiration

Post-transcriptional modifications such as adding a G cap and a poly-A tail to the newly
synthesized mRNA can be observed in - Answer-eukaryotes

What does the replisome not include? - Answer-Primers

What is true regarding rhodopsin-base phototrophy and a chlorophyll-base
phototrophy? - Answer-Rhodopsin-base phototrophy does not involve the electron
transport chain.

Chlorophyll vs bacteriorhodopsin - Answer-Chlorophyll is found in plants, algae, and
cyanobacteria. Electrons enter the electron transport chain.
Bacteriarhodopsin is found in bacteria and archaea and acts as a proton pump. Uses
proton motive force, NOT ETC

RNA polymerase recognizes the promoter region located on: - Answer-the DNA
molecule

Chaperones - Answer-dramatically increase in concentration when proteins are
exposed to high heat and adverse conditions to protect and refold proteins if necessary.

What are some final electron acceptors in anaerobic respiration? - Answer-SO4, NO3,
CO2

tRNA charging is - Answer-completed by aminoacyl-tRNA synthetases

What is the role of DNAj and DNAj in protein secretion? - Answer-To assist in folding
the protein

True or false: In translation 16S, rRNA acts as ribozyme during the elongation step -
Answer-False

What does 23S rRNA do? - Answer-Catalyze the formation of peptide bond between the
two amino acids

, Transcription of operons in procaryotes produces monocistronic mRNA or polycistronic
mRNA? - Answer-polycistronic. Eukaryotes produce monocistronic

What can cause the termination of RNA synthesis? - Answer-Stem loop structure in the
RNA molecule

Where does incoming amino acyl- tRNA bind to? - Answer-the A site

NO3 ----> N2 - Answer-Denitrification

What are true regarding protein folding? - Answer-Molecular chaperones are produced
in large quantities in response to thermal damage; DNAj and DNAk are examples of
heat-shock proteins which help in protein folding

What is a nucleosome? - Answer-The combination of DNA and proteins

Bacterial DNA is organized with help of ______ - Answer-chromatin-like protein

Are archaeal DNA and eukaryotic DNA similar? - Answer-YEP

What are consensus sequences in DNA transcription? - Answer-Prinbow box, TATA
box, -35 site

What is the Prinbow box? TATA box? - Answer-in bacteria, a series of TATAAT; found
in the promoter sequence and plays a role in initiating transcription. TATA is the same
thing but in eukaryotes

Which statement about the elongation of the lagging strand during DNA replication is
correct? - Answer-It requires a short RNA primer to proceed.

Polysomes are composed of - Answer-mRNA and ribosomes

DNA methylation - Answer-Used by E. coli mismatch repair system to distinguish old
DNA strands from new DNA strands. Old DNA strand is methylated

Fermentation involves the use of - Answer-substrate-level phosphorylation only

How does eukaryotic transcription differ from bacterial transcription? - Answer-
Eukaryotes have 3 major RNA polymerases; promoters differ from those in bacteria by
having a combination of many elements; eukaryotes have a cap, tail, and introns, and
eukaryotic genes are split or interrupted.

Which of the following is true regarding bacterial protein secretion? - Answer-Gram-
positive and gram-negative bacteria have different problems secreting proteins based
on the differences between the structure of their walls.

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