When using the oil immersion lens, what four procedures can be implemented to
achieve the maximum resolution? - ️️Following locating the specimen with the low
power or high-dry lens, change from low power lens or high-dry lens directly to oil
immersion lens. before putting immersion lens int...
Microbiology Lab Practicum #1 SBVC
When using the oil immersion lens, what four procedures can be implemented to
achieve the maximum resolution? - ✔️✔️Following locating the specimen with the low
power or high-dry lens, change from low power lens or high-dry lens directly to oil
immersion lens. before putting immersion lens into place, add a drop of mineral oil to
slide, then position oil immersion lens directly over slide, clicking it into place. open
diaphragm as much as possible and also place the condenser at it's highest point.
How do you clean culture spills in lab? - ✔️✔️a. Flood the area with disinfectant.
b. Cover with paper towels and let it sit for 15 minutes.
c. Notify the instructor or lab technician.
d. Alert students as to the location of the spill while paper towels are in place so as to
prevent anyone from slipping on it.
e. Wipe up the area with paper towels and place all towels in the biohazard container
f. Using a hand broom and dustpan, sweep up glass and dispose of it into a pipette bin
to keep sharp objects in a non-penetrable container.
Ocular lens - ✔️✔️Magnifies the object, usually by 10x. Also known as the eyepiece,
this is the part you look through to view the object
What are the objectives on microscope? - ✔️✔️scanning objective 4x, low power
objective10x, high dry power objective 40x, and oil immersion objective 100.
How do you carry your microscope? - ✔️✔️Handle the microscope carefully. Keep a
firm grasp on the arm and a protective hand under the base when transporting it from
the desk to the cabinet.
How do you put away your microscope? - ✔️✔️.1 Remove the slide from the stage and
clean any traces of immersion oil, stain, water, or other foreign substances from the
stage or any other part of the microscope.
2. Check that the blue filter is not extended.
3. Rotate the scanning objective lens into the "down" position. (red lens in front)
4. Raise the nosepiece or lower the stage to about the halfway point, creating a greater
working distance between the objective lenses and the stage.
5. Coil the power cord neatly around the hooks provided on the back of the scope
6. Be sure the head screw is tight so that the head is not wobbly.
7. Turn light all the way off, and turn off power.
8. Cover the microscope with its protective dust cover.
Substage light - ✔️✔️The light source is usually built into the base of the microscope
and typically has a dial or sliding bar on one side to control the light intensity.
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