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CRISPR LAB 2024 QUESTIONS WITH COMPLETE SOLUTIONS!!
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CRISPR LAB 2024 QUESTIONS WITH COMPLETE SOLUTIONS!!
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Name: Score:14 Multiple choice questions
Term 1 of 14
What was the role of Cas9 in our experiment?
To bind to the cell membrane and facilitate nutrient absorption.
To latch onto free floating genetic material, search for viral match to chop up and kill
To enhance the replication speed of the bacterial dna.
To act as a carrier for transporting proteins within the cell.
Term 2 of 14
What was the purpose of the CRISPR lab?
The purpose of the lab was to try to edit same e.coli to make them resistant to antibiotics
like streptomycin.
The purpose of the lab was to develop a method for cloning e.coli for research purposes.
The purpose of the lab was to study the effects of antibiotics on bacterial growth.
The purpose of the lab was to create a new strain of e.coli that produces insulin.
Term 3 of 14
What was the role of the template DNA in this lab?
It is used to amplify the dna before it is cut by cas9.
It is added to Cas9 so that cells can do homology directed repair and have an undamaged
gene.
It serves as a marker to identify successful gene edits.
It is a byproduct of the dna editing process that needs to be removed.
, Definition 4 of 14
-Cas9 protein: makes double strand breaks in the target DNA sequence
-Guide RNA: directs the Cas9 complex to a target sequence that can be designed to target your
sequence of interest
What was the purpose of the CRISPR lab?
What was the role of the template DNA in this lab?
Key Components of the CRISPR-Cas9 Editing Complex
Key Components of Plasmid
Definition 5 of 14
-Stands for clustered regularly interspaced short palindromic repeats (refers to specialized
region of DNA in bacteria with a series of short palindromic repeats)
-Uses Cripsr associated proteins (aka Cas proteins)
-This system functions in bacteria/archae as an "immune system" fighting viruses and other
infections by chopping up the DNA of the invader
-After chopping up the DNA, Crispr system inserts DNA tags from the invader into the Crispr
region to "remember" the invader ( just like B and T cells remember previous infections), if cell is
infected by that virus again, RNA from these sequence tags helps the bacteria recognize the
invader
-Scientists have developed technology using this natural process as a gene editor
Plates Used
CRISPR for Editing
CRISPR
Our CRISPR Lab
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