UAMS Hematology Lab Practical Final
Review *Q&A* (100% Correct) 2024/2025
|VERIFIED|
**What is the process for preparing a smear?**
Place a drop of blood from an EDTA tube about half an inch from the frosted end of a glass slide.
Lay the slide flat on a surface. Hold a second slide (the...
UAMS Hematology Lab Practical Final
Review *Q&A* (100% Correct) 2024/2025
|VERIFIED|
**What is the process for preparing a smear?**
Place a drop of blood from an EDTA tube about half an inch from the frosted end of a glass slide.
Lay the slide flat on a surface. Hold a second slide (the spreader) at a 30-45 degree angle with your
fingertips and place it in front of the blood drop. Pull the spreader slide back into the blood drop,
allowing the blood to spread across the width of the slide. Quickly and smoothly push the spreader slide
forward.
**What tools are needed to prepare a smear?**
Glass slides, blood samples, and cover slips.
**What causes a poor smear?**
Dirty slides, erratic motions, pointed feather edges, overly steep angles of the spreader slide,
insufficient blood spreading, high lipid levels, and unequal pressure.
**What is the purpose of a coverslip smear?**
Used for preparing bone marrow samples.
**What items are required for Wright's stain?**
Wright's stain, buffer, rinse solution, a timer, and containers for holding the stain, buffer, and
rinse.
**What sources of error can occur when staining a smear?**
Stains or buffers that are too acidic or alkaline, excessive staining time, using heparinized blood
samples, prolonged rinsing, and insufficient buffering.
**What is an ideal area to view on a blood smear?**
1
, Cells should be adjacent to one another with minimal overlap.
**How is a WBC estimate performed?**
Select a suitable area on the slide and average the number of white blood cells observed in five
low-power fields. Multiply the average by 200 (one white cell under low power, at 10x magnification,
approximates to 200 WBCs).
**How do you check for platelet clumping?**
After estimating WBCs, inspect the feather edge or side margins of the smear for platelet clumps.
**What could cause difficulty in focusing the slide?**
The slide may be upside down, oil was not applied to the slide, or the coarse adjust knob may
have been turned excessively in either direction.
**What constitutes a good area in oil immersion?**
Cells should be touching with minimal overlap, aiming for approximately 200 RBCs per oil field.
**What is examined under oil immersion?**
We assess the morphology of red blood cells, identify and differentiate white blood cells, and
evaluate platelets for quantity, size, and morphology.
**How is a platelet estimate conducted?**
Calculate the average platelet number in five oil fields and multiply the result by 20,000.
**What does an aged slide look like? How old is too old?**
Aged samples may show disintegrating WBCs, appearance of vacuoles, platelet clumping, and
variations in RBC size and shape, such as crenation. A sample is too old if it has been at room
temperature for more than 5 hours.
**What is the difference between agglutination and rouleaux?**
2
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