Steps of Recombinant DNA Technology Review Questions with Complete Solutions Rated 100%
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Steps of Recombinant DNA Technology
Institution
Steps Of Recombinant DNA Technology
Steps of Recombinant DNA Technology Review Questions with Complete Solutions Rated 100%
First Step - Answers Isolation of plasma DNa & DNA containing gene of interest
2nd Step - Answers Gene inserted into plasmid
3rd Step - Answers Plasmid put into bacterial cell
4th Step - Answers Cells clone...
Genetically manipulate corn for high yield & enhanced flavor
Genetically manipulate tomatoes for slower ripening
Identify criminals cases- murder or rape
DNA fingerprinting
Cytocrom C - Answers important electron carrier in respiratory pathaway, found in all aerobic
eukaryotes
Restriction endonucleases - Answers enzymes that have been purified from a different species of
bacteria and restriction enzyme
Restricting/digesting - Answers Cutting requires energy in form of ATP, involves physical cleaving of
chemical bonds,
specific recognition, sites where cuts occur and are palodromic
Plasmid - Answers Lab, Puc19 and E.Coli, relatively small extrocromisonal, circular molecule of DNA,
found in bacteria and yeast
Puc19 used for cloning because of size and DNA sequence
Agacrose gel - Answers Used to separate and sort fragments of DNA by size only, DNA fragments placed
in gel, electric current runs through matrix, fragments move at different rates depending on protein's
size and charge
Steps for cloning a gene and bacterial plasmid - Answers 1. Isolate DNA from two sources
2. Cut both DNA with some restriction enzyme
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