BIO 353 Exam 1 Study Guide with Complete Solutions
0 view 0 purchase
Module
BIO 353
Institution
BIO 353
BIO 353 Exam 1 Study Guide with
Complete Solutions
The Cell - Ans:-Basic unit of life
What are the major organelles/structures of a basic eukaryotic animal cell? - Ans:-Nucleolus,
nucleus, nuclear envelope, nuclear pore, chromatin (DNA), mitochondria, endoplasmic reticulum, golgi
complex, acti...
Describe optical tweezers and how they can be used. - Ans:✔✔-Instrument that uses a focused laser
beam to provide an attractive or repulsive force, depending on the refractive index mismatch to
physically hold and move microscopic objects
Can the cells be alive when using phase contrast? - Ans:✔✔-Yes
Describe the basic idea of epifluorescence microscopy - Ans:✔✔-The use of a fluorescence microscope
allows detection of specific proteins or other molecules/ions/organelles that are made fluorescent by
coupling them to a fluorochrome marker
Explain the steps involved in Indirect Immuno-fluorescence Microscopy - Ans:✔✔-1. Cells/tissues are
fixed (killed)
2. Exposed to the primary antibody, the antibody binds only to a specific protein it recognizes
3. Exposed to secondary antibody, the antibody binds only to the primary antibody. The secondary
antibody is coupled to a fluorochrome marker; thus location of protein in cells/tissues are fluorescently
labeled and may be viewed w/ a fluorescence microscope
Are the cells alive or dead for Indirect Immuno-fluorescence microscopy. - Ans:✔✔-Dead
What is GFP and how is it coupled to the proteins in different cellular structures? - Ans:✔✔-Green
Fluorescent Protein; it is inserted into the protein
Page 3/26
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller GraceAmelia. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for £9.12. You're not tied to anything after your purchase.
