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BIO 219 Quizzes: Questions With Solutions (Expert Verified)
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BIO 219 Quizzes: Questions With Solutions (Expert Verified)
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BIO 219 Quizzes: Questions With Solutions (ExpertVerified)
In the Hawaiian bobtail squid (Euprymna scolopes) what is the benefit to the
squid in hosting the Aliivibrio?
Communication
b.
Attracting a mate
c.
Attracting prey
d.
Hiding from predators Right Ans - d
Wash Buffer 2 (AW2) contains ________________ to remove the salts added from
the first wash buffer.
a.hydrochloric acid
b.SDS
c. Guanidinium chloride
d. Ethanol
e. sodium benzoate Right Ans - d
Which gene in the lux operon encodes the auto-inducer, AHL
Lux R
b.
Lux I
c.
Lux A
d.
Lux D Right Ans - b
What is the overall purpose of the quarter-long project?
I. To map the lux operon
II. To isolate the lux operon from A. fischeri and transform E. coli with it the
operon to induce bioluminescence
III. To induce the upregulation of the gadA gene in E. coli through quorum
sensing Right Ans - 2 and 3
,Regulation of gene expression in response to changes in cell population
density is known as _______________________. Right Ans - quorum sensing
If you wanted to get a brighter bioluminescence from your culture of Allivibrio
which of the following "treatments" would increase the amount of blue-green
light emitted?
treatment with CO2
b.
Treatment with chaotropic salts
c.
treatment with O2
d.
treatment with acid
e.
Treatment with a alkaline buffer Right Ans - c
What is used to elute the DNA from the silica membrane in the spin column?
a.
Chaotropic salts
b.
SDS (sodium dodecy sulfate)
c.
Water
d.
Ethanol
e.
A low pH buffer. Right Ans - c
Good minions always wear their [x] and [y] for lab safety! Right Ans -
gloves
goggles
What is the range for the 260:280 ratio that indicates a DNA sample of "good
purity"?
2.2 - 3.5
b.
0.5 - 1.5
c.
, 2.0 - 2.2
d.
1.5 - 1.8
e.
1.8 - 2.0 Right Ans - e
Of the assignments listed in the answers, which one is NOT done as group
work?
Final Exam
b.
Lab Report 1
c.
Lab Notebooks
d.
Lab Report 2 Right Ans - final exam
A 260:280 ratio = 2.36 would indicate protein contamination. Right Ans -
false
___ dissociates in the presence of chaotropic salts in the lysis buffer and forms
a cation bridge to bind DNA to the silica membrane in the spin column.
Right Ans - SDS
If you wanted to clone a seuqence that was 35-35 kb in size, which vector
would you choose?
a.
Bacterial Artificial Chromosome (BAC)
b.
Phagemid
c.
Bacteriophage vector
d.
Cosmid vector
e.
Plasmid vector Right Ans - D
Most restriction enzymes recognize specific [x] sequeces that are most
commonly [y] base pairs in length. Right Ans - palindromic
6
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