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Bio 219 Quizzes: Questions With Complete Solutions
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Bio 219 Quizzes: Questions With Complete Solutions
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Bio 219 Quizzes: Questions With Complete SolutionsWhich of the following is NOT a goal for our project this term in Bio 219?
a. To induce the upregulation of the gadA gene in E. coli through quorum
sensing
b.To map the lux operon
c. To transform E. coli with it the lux operon to induce bioluminescence
d. To isolate the lux operon from Aliivibrio fischeri Right Ans - B. To map
the lux operon
Which gene in the lux operon encodes for the transcriptional activator that
binds the auto inducer?
a. Lux E
b. Lux A
c. Lux D
d. Lux R
e. Lux I Right Ans - D. Lux R
The Aliivibrio fischeri strain we are using the term (MJ 1741) comes from the
pineapple fish (Monocentris japonica). Also known as the pineconefish, it is
yellow with distinct large scales outlined in black. The fish is nocturnal and
feeds at night. During the day, it shelters in caves and under ledges. It has
light-producing organs filled with the bioluminescent bacteria on each side of
its lower jaw. Though the purpose of these photo-organs is not specifically
known, what do you think may be the benefit to the host organism?
a. Attracting prey
b. Attracting a mate
c.Hiding from predators
d.Communication Right Ans - A. attracting prey
High level of [x] activates the operon systems of luminous bacteria like A.
fischeri in its environment. The regulatory response to this molecule leads to
the induction of expression of the lux genes which is termed [y]. Therefore,
where is the bacteria is most likely to glow? Inside its host or in the open
ocean? [z] Right Ans - x: autoinducer (AHL)
y: quorum sensing
z: inside its host
What is used to elute the DNA from the silica membrane in the spin column?
,a. SDS (sodium dodecyl sulfate)
b.Water
c.Ethanol
d.A low pH buffer
e.Chaotropic salts Right Ans - B. water
What is the range for the 260:280 ratio that indicates a DNA sample of "good
purity"?
a.0.5 - 1.5
b.2.0 - 2.2
c.2.2 - 3.5
d.1.8 - 2.0
e.1.5 - 1.8 Right Ans - D. 1.8-2.0
A 260:280 ratio = 1.66 would indicate protein contamination. (True/False)
Right Ans - True
If you wanted to get a brighter bioluminescence from your culture of Aliivibrio
which of the following "treatments" would increase the amount of blue-green
light emitted?
a.Treatment with acid
b.Treatment with O2
c.Treatment with CO2
d.Treatment with chaotropic salts
e.Treatment with a alkaline buffer Right Ans - B. Treatment with 02
Wash Buffer 1 (AW1) contains low amounts of ________________ that binds to and
removes the proteins and colored contaminants.
a.Ethanol
b.SDS
c.Guanidinium chloride
d.Sodium benzoate
e.Hydrochloric acid Right Ans - C. Guanidinum Chloride
The Resuspension Buffer contains ____________ that can solubilize the
membrane proteins and disrupt the lipid bilayer of gram-negative cells to help
get the proteins into solution. The phospholipids in the membrane are
solubilized by this reagent. It also helps release the DNA by binding proteins
(both membrane and non-membrane proteins) and denaturing them..
, A. SBS (sodium benzoate salt)
b.Ethanol
c.Silica
d.SDS (sodium dodecyl sulfate)
e.Chaotropic salts Right Ans - D. SDS (sodium dodecyl sulfate
On the spin column, the formation of an anionic bridge allows DNA to bind to
the silica membrane in the column. (True/False) Right Ans - False
The maximum wavelength of DNA absorbance is 260nm, within the UV range.
This is due to the nitrogenous bases of DNA (adenine, cytosine, guanine, and
thymine). At this wavelength, double stranded DNA (dsDNA) has a higher
absorbance the ssDNA due to having more nitrogenous bases. (True/False)
Right Ans - False
What is the role of restriction enzymes, in vivo?
a.To restrict viral DNA from entering the cell
b.To ward off viral DNA by making the membrane impermeable
c.To act as a bacterial defense system against viral DNA
d.To enhance the uptake of viral DNA Right Ans - C. To act as a bacterial
defense system against viral DNA
When cutting the genome of A. fischeri with Sal I you should produce, on
average, _______________ sized fragments.
a.12 kb
b.9 kb
c.8.5 kb
d.4 kb
e.6 kb Right Ans - b. 9 kb
Which of the following make pGEM a desirable vector for our study?
I. Dual promoter system
II. Multiple cloning site
III. Selectable markers Right Ans - I, II, and III (all)
Only chromosomal DNA (chDNA) when compared to plasmid DNA, exists in a
supercoiled state when undisturbed in a cell. (True/False) Right Ans - False
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