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Ch10 Genetic Engineering and Recombinant DNA Exam Questions with Complete Answers
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Ch10 Genetic Engineering and Recombinant DNA Exam Questions with Complete Answers
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Ch10 Genetic Engineering andRecombinant DNA Exam Questions with
Complete Answers
The primary intent of ... DNA technology is to combine the genetic material from two
organisms - Answer-recombinant
The area of study specifically focuses on the production of all proteins produced by an
organism at a given point of time - Answer-proteonomics
Cloning hosts commonly used in recombinant DNA technology include: - Answer-
Several bacterial species, yeasts, animal cell cultures, live animals and plants
Put the three basic steps of the polymerase chain reaction (PCR) in order - Answer-1.
Denaturation, 2. Priming, 3. Extension
True or false: The advantages of cDNA in genetic engineering is the synthesis of
eukaryotic genes without introns - Answer-True
There are thousands of restriction enzymes (endonucleases) that cut ... at a different
sequence. - Answer-DNA
How are restriction enzymes named? - Answer-1. First letter of the bacterial genus 2.
First two letters of the bacterial species. 3. Number showing the order discovered in the
bacterium.
CRISPR is a system for genetic engineering that has been found in - Answer-archaea
and bacteria
high throughput sequencing - Answer-PCR
whole genome shotgun sequencing - Answer-cloning
True or false: Gel electrophoresis is a laboratory technique for separating DNA
fragments according to length by employing electricity to force the DNA through a gel
matrix - Answer-True
... is most often used in the laboratory to separate strands of DNA - Answer-Heat
True or False. Humans make good cloning hosts - Answer-False
DNA ... is also known as DNA typing or profiling - Answer-fingerprinting
, Which make good cloning vectors? - Answer-bacteriophages and plasmids
... ... is the enzyme that can convert RNA to DNA - Answer-reverse transcriptase
Who was the first person to create a living, self-replicating cell synthesized from
chemicals? - Answer-Venter
whole-genome shotgun sequencing steps - Answer-1. Break DNA into small fragments.
2. Separate the DNA by size in gel electrophoresis.
3. Clone the DNA into plasmid vectors.
4. Generate DNA sequence from the clones.
5. Assemble the DNA sequence into larger contigs.
6. Edit the sequence to remove any errors.
DNA fingerprinting is used for - Answer-tracing lineage of organisms, identifying
microorganisms, crime scene investigations, paternity testing, and detecting genetic
diseases.
True or false: Some diseases are actually due to inappropriate expression or over
expression of a gene. - Answer-true
What are the steps of Polymerase Chain Reaction (PCR)? - Answer-1. Denaturation. 2.
Priming. 3. Extension
Several Pseudomonas species have been genetically altered creating strains that have
which of the following characteristics? - Answer-Ability to produce insecticide, inability to
form ice crystals
The pieces of DNA produced by restriction endonucleases are termed... ... - Answer-
restriction fragments
Which type of molecule can silence gene expression, and whose levels can be modified
in order to treat disease expression? - Answer-miRNA
Gene probes are commonly used for - Answer-- Identifying bacteria or viruses in an
unknown culture.
- Diagnosing causative agents of infection.
A ... ... is a collection of DNA clones that represent the entire genome of an organism -
Answer-genomic library
multicloning site (MCS) - Answer-A region of DNA on a cloning vector that has a
number of restriction enzyme recognition sequences to facilitate the cloning of a gene.
Steps for Recombinant DNA - Answer-1. Remove and isolate a selected gene from a
donor organism
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