BIO 340 Module 5 Exam Questions And Answers 100% Pass
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Module
Bio 340
Institution
Bio 340
BIO 340 Module 5 Exam Questions And
Answers 100% Pass
What is the correct order of enzyme action during DNA replication? Number the steps from 1 to 7. -
answer1. Binding to origin of replication (AT rich sequence)
2. Helicase/topoisomerase activity
3. Single-stranded binding proteins bind and
...
BIO 340 Module 5 Exam Questions And
Answers 100% Pass
What is the correct order of enzyme action during DNA replication? Number the steps from 1 to 7. -
answer✔1. Binding to origin of replication (AT rich sequence)
2. Helicase/topoisomerase activity
3. Single-stranded binding proteins bind and
prevent reannealing
4. Synthesis of RNA primers (priming)
5. DNA polymerization/proofreading
6. 5'-3' exonuclease activity/DNA polymerization
7. Ligation
Transcribe and Translate the following DNA strand
DNA 3' T A C A T A A C C A T T 5' - answer✔5' AUGUAUUGGUAA
The haploid human genome is 3 Gbp long (3x10^9 bp). How long would the genomic DNA in a single
diploid cell in your body be if it were stretched out end to end? - answer✔-2m
The haploid human genome is 3 Gbp long (3x10^9 bp). There are approximately 50 trillion cells in the
human body. How long would all of the human genomic DNA in your body be if it were stretch out end
to end? - answer✔100 billion Km
At what temperature range does a DNA allow the binding of primers? - answer✔45-68
What are the three main components of a DNA nucleotide? - answer✔A phosphate group, a
nitrogenous base, a deoxyribose
Hydrogen bonds are weaker than covalent bonds. During PCR, hydrogen bonds are broken during the
denaturation step. - answer✔true
T or F regarding DNA Adenine pairs with Uracil. - answer✔False
T or F Two strands of DNA Double helix are held together by a weak bond. - answer✔True
You are running a PCR and want to estimate about how much DNA you could have after 30 cycles on the
thermocycler. - answer✔2.14 x 109
What are all the necessary reagents required for the PCR - answer✔1. Double stranded DNA complex
with target sequence
2. Deoxyribose nucleoside triphosphate
3. Heat stable DNA polymerase
4. two nonidentical single strand DNA priming sequences (forward and reverse)
5. Buffer
Denaturation - answer✔heat the mixture to 95° C this will separate the double stranded DNA into two
single strands
Primer Annealing (PCR) - answer✔the reaction temperature is lowered to 48-68° C. This allows primers
to create bonds with their complementary sequences of the target DNA
Extension/ Elongation - answer✔the temperature is raised to 72° C. This allows polymerase to
synthesize DNA accurately and rapidly
the process where the 3' hydroxyl group of the growing chain and the 5' phosphate of a free nucleoside
triphosphate react to form a phosphodiester bond. 5' looses two phosphate to form this bond. The
directionality is important because if these strands are not running antiparallel the phosphodiester bond
will not form. - answer✔Elongation
Frederic Griffith: bacterial transformation - answer✔the R-strain bacteria must have taken up what he
called a "transforming principle" from the heat-killed S bacteria, which allowed them to "transform" into
smooth-coated bacteria and become virulent
The Avery-MacLeod-McCarty Experiment - answer✔demonstrated that DNA is the genetic material
because degradation of DNA led to a cessation of bacterial transformation
The Hershey-Chase experiment - answer✔32P found in pellet, 35S found in supernatant
Structure of DNA - answer✔It is composed of four kinds of nucleotides, joined
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