Biochemistry ACS Final Exam Questions
And Answers (Guaranteed A+)
What is the Henderson-Hasselbach ? - answer✔pH = pka + log ([A]/[Ha])
High Ka, low pka indicates what? - answer✔Strong acid
What is the relation between pH and [H+]? - answer✔pH = -log[H+]
What is the realtionship between pka and Ka? - answer✔pka = -logKa
What makes a good buffer? - answer✔A weak acid mixed with its conjugate base in a 50/50
ratio
What destroys tryptophan? - answer✔Acids and bases
What is the isoelectric point? - answer✔The pH in which a particular molecule carries no net
charge (pI)
Side chain pka of aspartic acid? - answer✔~ 3.86
Side chain pka of arginine? - answer✔~ 12.48
Side chain pka of cysteine? - answer✔~8.0
Side chain pka of glutamic acid? - answer✔~4.07
Side chain pka of histidine? - answer✔~6.1
Side chain pka of lysine? - answer✔~10.53
Side chain pka of tyrosine? - answer✔~10.00
Inductive effect on pka? - answer✔Electronegative atoms pull electrons away from another
group, and makes that particular groups more positive. Generally lowers pka
Electrostatic effect on pka? - answer✔Nearby positive charge makes a proton want to leave
more, generally lowers pka.
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