Summary
Summary Topic 6 DNA profiling
Edexcel Biology Topic 6 DNA Profiling, Forensics, Gel Electrophoresis, Southern Blotting, PCR
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DNA Profiling23 000 =
90000 proteins
genes
.
Exons lutons
genes
are made
of and
S.I units
Exons
coding
=
Introns
coding
=
non
Es 12 En 12 Es Is
↳
different combinations of Exons
different codon
-
sequence
leng he
of mRNA
-
-
= different amino
Sequence dcid
different polypeptide laught
Spliceosome cuts out introns
through post
transcriptual splice up
between Exors
reforms phosphodiester bonds
↳
Post translation the
purpose of
the
modification is
golgi .
Introns identifiable characteristics molecular
for
-
phylogeny and DNA
profiling
STR's >
- Short Tandem repeats (2-50 bases)
base repeat
e .
.
g agtaagtaagta-4
alleles have
different number
of repeats
↳
, Maternal and Paternal chromosomes
may af
of repeats
different
have number STR
na same
gene 102i (homologous pairs)
E #
S.I units
7 repeats a
repeats
Principles and rationale of DNA profiling
.
1 DNA
Everyones is
unique
.
2 Except (identical)
twins
in
monozygotic
3 . Based on short tandem
repeats in Introns
.
4.
Compare bands of DNA (DNA profile) gel electrophesis
5 10 loci compared to be reliable
gene
.
Forensics
-
find tissue
- hair
,
blood
DNA break
from tissue membranes
->
>
down and
-
histore proteins
. DNA
1
may
be
partially
Contamination
decomposed
DNA
of
.
2
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