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Laboratory Procedure to Analyze The DNA
- Institution
- Northumbria University (UNN)
This assignment describes the real practical procedure of DNA extraction, PCR procedure and gel electrophoresis of human DNA.
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Table of contentContents Page number
1. Introduction ………………………………………………………………5
2. Objective ……………………………………………………………....7
3. Materials ……………………………………………………………………………………………………. 7
4. Method
4.1 Subject ………………………………………………………………8
4.2 Blood sampling ………………………………………………………8
4.3 Isolation of DNA from blood sample ………………………………9
4.4 Estimating the concentration of genomic DNA
4.4.1. Preparation of agarose gel ………………………………………10
4.4.2. Quantifying and quality analysis of DNA ……………………....11
5. Result ………………………………………………………………………12
6. Discussion ………………………………………………………………13
7. Appendix ………………………………………………………………16
8. References ………………………………………………………………18
1|Page
, List of figures
Figure 1. DNA structure ……………………………………………………………………..5
Figure 2. Refrigerated centrifuge machine ……………………………………………..6
Figure 3. Venepunture procedure ……………………………………………………………..8
Figure 4. DNA extraction observed in pellet ……………………………………………..9
Figure 5. Extracted DNA washed by 70% ethanol ……………………………………10
Figure 6. DNA in the eppendorf tube after washing …………………………………....10
Figure 7. Agarose gel preparation method …………………………………………....11
Figure 8. Movement of Sample DNA and different DNA markers on the gel visualized by UV
light in quantum S4 ……………………………………………………………………………12
2|Page
, List of tables
Table 1. Different concentration markers of different DNA samples ……………………11
Table 2. Different dyes to indicate different sizes of DNA ……………………………………14
3|Page
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