Immunohistochemistry - Study guides, Revision notes & Summaries

PATH2220 Final Exam - Learning outcome- based Questions and Answers 2024 define key terms associated with the pathological basis of disease such as disease, aetiology, pathogenesis - Answer- Disease = abnormal condition where normal function (homeostasis) of body part/organ is disturbed Aetiology = cause of disease/condition (inherited/acquired) Pathogenesis = manner of development of disease - cellular events / changes / reactions caused by aetiology that alter normal cellular processes ...

What does hematoxylin and eosin stain? Correct Answer-Hematoxylin (purple) stains basophilic structures. Examples: DNA, RNA, Ribosomes Eosin (pink) stains eosinophilic/acidiphilic structures. Examples: cytoplasm and collagen. Which stain detects carbohydrates? What color does it turn carbs? Correct Answer-Periodic Acid-Schiff Reactions stain carbohydrates magenta Alcohols and turn them into doubled bonded oxygens (aldehydes). Schiff reagent reacts w/aldehyde to form the magenta color. ...

NRSC 2150 Exam 1 Review Questions and answers, VERIFIED/ how are different classes of neurons identified? via gene expression --> PROTEINS make a cell specific Criteria for a neurotransmitter 1. molecule must be synthesized & stored in the presynaptic neuron 2. molecule must be released by the presynaptic axon terminal upon stimulation 3. molecule must produce a response in the postsynaptic cell that mimics the response produced by the release of neurotransmitter from the presynapti...

EBME 306 - Unit 1 - Immunocytochemistry techniques (Advantages and Disadvantages) 100% Correct EBME 306 - Unit 1 - Immunocytochemistry techniques (Advantages and Disadvantages) 100% Correct EBME 306 - Unit 1 - Immunocytochemistry techniques (Advantages and Disadvantages) 100% Correct EBME 306 - Unit 1 - Immunocytochemistry techniques (Advantages and Disadvantages) 100% Correct Given immunocytochemistry technique List: 1. Advantages 2. Disadvantages 3. Applications - ANSWER Immunohist...

What stain that makes iron identifiable? - Answer-Prussian Blue What element makes the fixation process more difficult by requiring an added acid step? - AnswerCalcium What stain is typically used for a cytology? - Answer-Romanovsky Stain What stain could be used to identify rabies? - Answer-IHC (Immunohistochemistry) What compound are misfolded proteins tagged with to mark them for destruction? - AnswerUbiquitin What structure helps lysosomes maintain a low lumen pH? - Answer-Proton pumps ...

Given immunocytochemistry technique List: 1. Advantages 2. Disadvantages 3. Applications - ANSWER Immunohistochemistry and immunocytochemistry - ANSWERAdvantages: Specific localisation of antigens in situ. Staining lasts for years. Relatively inexpensive- no specialised equipment required. Disadvantages: Not suitable for light impenetrable materials. Qualitative, and subjective without computer analysis. Application: Commonly used for the examination of pathological samples e....

PATH2220 Final Exam - Learning outcome- based Questions and Answers 2024 define key terms associated with the pathological basis of disease such as disease, aetiology, pathogenesis - Answer- Disease = abnormal condition where normal function (homeostasis) of body part/organ is disturbed Aetiology = cause of disease/condition (inherited/acquired) Pathogenesis = manner of development of disease - cellular events / changes / reactions caused by aetiology that alter normal cellular processes ...

toplasmic polarization signaling proteins commonly divided into three groups called the Par, Scribbled, and Crumbs complexes, and activation of the small GTPases Rac1 and Cdc42 (Nelson 2009). The extrinsic polarization mechanism, on the other hand, provides spatial orientation cues to the cell from the environment, triggering the asymmetric distribution and activation of the complexes that make up the intrinsic mechanism. In early embryos, primary spatial cues take a variety of forms. ...

Acytelcholine - Answer--Excitatory; increases muscle contraction and depresses parasympathetic system. Associated with learning, memory, and attention → too little leads to Alzheimer's -Basal forebrain→ contains nucleus basalis -Targeted by Nicotine and succinylcholine Adaptation - Answer-The slowing of action potential firing overtime -Occurs in K+ Hyperpolarization Channels Agonist vs. Antagonist - Answer-Agonist: Chemical binds to the same site as an endogenous substance (e.g., ne...

OSSF - Exam 1 Questions and Answers 100% Pass What stain that makes iron identifiable? - Correct Answer ️️ -Prussian Blue What element makes the fixation process more difficult by requiring an added acid step? - Correct Answer ️️ -Calcium What stain is typically used for a cytology? - Correct Answer ️️ -Romanovsky Stain What stain could be used to identify rabies? - Correct Answer ️️ -IHC (Immunohistochemistry) What compound are misfolded proteins tagged with to mark them ...