Methylation - Study guides, Revision notes & Summaries

When explaining genetic coding to a group of students, the instructor discusses gene activation and deactivation. It was stressed that inactivation of a gene requires which of the following processes? Correct Answer-Methylation of histone amino acid When an infant is born with gene mutations in his cells, the nurse explains to the parents that accidental errors may be a result of: Select all that apply. Correct Answer-Loss of one or more base pairs Substitution of one base pair for another ...

histones are rich in which 2 AA's - correct answer lysine & argenine Barr bodies (what, type of chromatin) - correct answer inactive X chromosomes heterochromatin (highly condensed, sterically inaccessible) less condensed, transcriptionally active, sterically accessible chromatin - correct answer euchromatin DNA methylation at CpG islands - correct answer represses transcription "CpG Methylation makes DNA Mute" DNA methylation (what is methylated and why?) - correct answer tem...

ASCP MB: Extra-Practice Questions & Answers 2024 The Klenow fragment of E. coli DNA polymerase I is used: - ANSWER-to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - ANSWER-gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucle...

1. which of the following statements about chickens is false? a- all chickens perceive danger equally b-genetics pre-determines how a chicken responds to danger c-a stress resilient chicken is able to recover from danger and remain healthy d-all of the above ANS: a 2. which of the following could contribute to variation in the stress response of lambs at weaning? a-methylation of the glucocorticoid receptor gene b-polymorphisms in the melancortin 2 receptor gene c-polymorphisms in the nic...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...

Angelman Syndrome causes (expression and % of total) correct answers **paternal imprinting defect, should be maternal expression 5-7 kb deletion on maternal chr15q11.2-13 (60-70%) UBE3A maternal deletion (11%) Paternal UPD15 (3-7%) Angelman testing strategy and condition features correct answers methylation first since finds ~80% of cases then UBE3A seq and del/dup features: happy demeanor, abnormal gait, seizures, delayed/absent speech Prader-Willi Syndrome causes (expression and...

ABGC Boards Overview Questions and Answers 100% Solved Angelman Syndrome causes (expression and % of total) ️️**paternal imprinting defect, should be maternal expression 5-7 kb deletion on maternal chr15q11.2-13 (60-70%) UBE3A maternal deletion (11%) Paternal UPD15 (3-7%) Angelman testing strategy and condition features ️️methylation first since finds ~80% of cases then UBE3A seq and del/dup features: happy demeanor, abnormal gait, seizures, delayed/absent speech Prader-Wil...

Genetics Exam #2 With Questions and Answers 100% Solved What is the molecular mechanism for imprinting a gene? a. acetylation b. methylation c. phosphorylation d. mutation e. nitration - answerB Genomic imprinting most commonly uses____ to ____genes. Select one: a. acetylation : activate b. methylation : activate c. phosphorylation : activate d. methylation : deactivate e. phosphorylation : deactivate - answerD Individuals with three copies of most autosomes do not survive. Indivi...

When water-soluble vitamins are consumed in excess of body needs, generally the excess is: a. excreted in the feces. b. stored in liver, bone, and adipose tissue. c. excreted in the urine. d. not absorbed. correct answers C A major function of B vitamins is to serve as: a. antibodies. b. a source of energy. c. a part of a coenzyme. d. electrolytes. correct answers C Most animals synthesize their own ascorbic acid from _____. a. glucose b. glutamine c. glycogen d. alanine correc...

B form DNA correct answersRight handed, 10.5 bp Lysine (K) and Arginine (R) correct answersAssociated with Histones Bromophenol blue correct answersTracking dye Capillary electrophoresis correct answersMust be ss PFGE buffer correct answersTBE Store RNA > 7 months correct answers-70 ethanol Branched DNA correct answersDNA/RNA targets Methylation or FISH correct answersPWS/AG Nested PCR correct answers2nd step, more specific Denaturation HPLC correct answersDetecting...