Fluorescent proteins - Study guides, Revision notes & Summaries

Why did the researchers attach a fluorescent dye to the membrane proteins? - Answer-to be able to visualize the proteins in the plane of the membrane After application of the laser, the bleached spot slowly regains fluorescence over time, so the researchers were able to conclude that: - Answer-proteins move in the plane of the membrane. In the graph, what does the

BIO 353 Exam 1 Study Guide with Complete Solutions The Cell - Ans:-Basic unit of life What are the major organelles/structures of a basic eukaryotic animal cell? - Ans:-Nucleolus, nucleus, nuclear envelope, nuclear pore, chromatin (DNA), mitochondria, endoplasmic reticulum, golgi complex, actin filaments, microtubule, peroxisome, ribosomes in cytosol, intermediate filaments, lysosome, vesicles, centrosome w/ pair of centrioles, plasma membrane, extracellular matrix What are the three majo...

What are the different types of microscopy and staining and when are they used? CORRECT ANSWER Types of microscopy include: light microscopy- used primarily to get an idea of the form of the bacteria, used in combination with staining to get an idea of Gram positive and Gram negative protein. Green fluorescent is used to observe cell occurrences like binary fission and movement of proteins. Electron microscopy is very detailed at getting an idea of what is occurring inside and outside of the ce...

What class device would a 'Drug Eluting Arterial Stent' be classified as? A) Class II device B) Class I device C) Class III device D) Class IV device - ANSWERD) Class IV device A suitable staining agent for a blood smear is: A) Gram's Stain B) Masson's Trichrome Stain C) H&E Stain D) Wright's Stain - ANSWERD) Wright's Stain In the context of biomaterials, the FDA regulates: A) Metals and Polymers B) Ceramics and Polymers C) Any material invented in a lab and test in vitro ...

BIOL 266 Final Exam 2024 Actual Questions and Answers Graded A   BIOL 266 Final Exam 2024 Actual Questions and Answers Graded A = Correct answer = We don’t have the answer Exam 1: MULTIPLE CHOICES: 1. You have isolated a mutant line of mammalian cells deficient in emerin. This mutant line is expected to be impaired in: A. Export of 40S and 60S ribosomal subunits from the nucleus B. Ribosome assembly in the nucleolus C. Transcription of some genes in certain ti...

BIO 353 Study Guide exam 1 with Complete Solutions The cell - Ans:-The basic unit of structure and function in living things What are the major Organelles/ structures of a basic eukaryotic cell? - Ans:-nucleus nucleolus ribosomes smooth ER rough ER mitochondria What are the 3 major disciplines involved with studying biology? - Ans:-bioimaging biochemistry mol. biology/genetics ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 2/22 What is l...

cytoplasmic organelles are: a. absent in prokaryotic cells and present in eukaryotic cells. b. present in both prokaryotic and eukaryotic cells. c. present in prokaryotic cells and absent in eukaryotic cells. d. absent in both prokaryotic and eukaryotic cells. correct answers a. absent in prokaryotic cells and present in eukaryotic cells E. coli is a useful model system for molecular biology studies because: a. it has a small genome. b. it reproduces rapidly. c. mutants can easily be i...

In a fetus, blood is shunted from the pulmonary artery to the aorta via what structure? - Answer- Ductus arteriosus What hormone stimulates uterine contraction - Answer- Oxytocin What age is it vital to avoid painful/traumatic experiences - Answer- 8-10 weeks What internal parasite is a threat to young (but typically not adult) horses - Answer- Parascaris equorum After parturition, it is normal for the bitch to have a reddish brown vaginal discharge for a few weeks, what is this call...

D103 Review study Guide Questions with Complete Solution 2024/2025 Update Principles of immunofluorescence staining we want to identify 2 dif proteins, how do we do it? *Make sure that ........ (2 essential parts to note)First we must generate primary antibodies that recognize/bind our target protein from 2 DIFFERENT species animals. then, use secondary antibody (tagged with color) from a 3rd animal that recognizes/binds the primary but is a dif color than the other secondary antibod...

What does hematoxylin and eosin stain? Correct Answer-Hematoxylin (purple) stains basophilic structures. Examples: DNA, RNA, Ribosomes Eosin (pink) stains eosinophilic/acidiphilic structures. Examples: cytoplasm and collagen. Which stain detects carbohydrates? What color does it turn carbs? Correct Answer-Periodic Acid-Schiff Reactions stain carbohydrates magenta Alcohols and turn them into doubled bonded oxygens (aldehydes). Schiff reagent reacts w/aldehyde to form the magenta color. ...