Recombinant Study guides, Revision notes & Summaries

Why is it important to use the same restriction endonuclease on DNA samples in an experiment? CORRECT ANSWER Access same base sequence So get fragments with required gene **Suggest why scientists insert a plasmid with EGFP (a gene that codes for bright protein) CORRECT ANSWER Those cells that do not glow brightly have not taken up the plasmid Name the enzyme used to cut a gene from human DNA CORRECT ANSWER Restriction endonuclease Suggest reasons why very few live births result from t...

This is a comprehensive and detailed note for Biol 375 that focuses on Recombinant DNA technology and applications. U'll need it!!

Describe the five general procedures in DNA cloning Correct Answer-1. Obtaining the DNA segment to be cloned 2. Selecting a small molecule of DNA capable of autonomous replication 3. Joining two DNA fragments covalently 4. Moving recombinant DNA from the test tube to a host organism 5. Selecting or identifying host cells that contain recombinant DNA Are restriction enzymes made by eukaryotes or prokaryotes? What is their function in vivo? Correct Answer-Restriction endonucleases are found...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...

Principles of Biochemistry 4th Edition Testbank(Horton) Chapter 1 Introduction to Biochemistry Chapter 2 Water Chapter 3 Amino Acids and the Primary Structures of Proteins Chapter 4 Proteins: Three-Dimensional Structure and Function Chapter 5 Properties of Enzymes Chapter 6 Mechanisms of Enzymes Chapter 7 Coenzymes and Vitamins Chapter 8 Carbohydrates Chapter 9 Lipids and Membranes Chapter 10 Introduction to Metabolism Chapter 11 Glycolysis Chapter 12 Gluconeogenesis, The Pentose Pho...

Summary of the content covered in the recombinant DNA technology module of MCB2021F

ATI RN Nursing Care of Children Online Practice 2019 B with NGN A nurse is caring for a newly admitted school-age child who has hypopituitarism. Which of the following medications should the nurse expect the provider to prescribe? Desmopressin Luteinizing hormone-releasing hormone Recombinant growth hormone Levothyroxine Recombinant growth hormone Recombinant growth hormone injections are used to treat hypopituitarism, which inhibits cell growth and results in growth failure. The nurs...

Pharmacology Exam 3: Endocrine System Drugs + NCLEX QuestionsSTART OF PITUITARY DRUGS - correct answer START OF PITUITARY DRUGS Pituitary Drugs -- List the 4 anterior pituitary drugs? + mechanism of actions - correct answer >cosyntropin MOA: stimulate secretion of cortisol from adrenal gland >somatotropin MOA: recombinant GH >somatrem MOA: recombinant GH >octreotide MOA: inhibits GH release Pituitary Drugs -- List the 2 posterior pituitary drugs? + mechanism of actions...

WGU D027 Course Material Questions and Answers Latest Update, Graded A A 23-year-old woman comes in for prenatal counseling. While completing her family history, she reports her brother has cystic fibrosis. She does not know if she is a carrier. She asks if her children will be affected by the disease. What is an accurate way to determine the likelihood of this patient's children being affected? Correct Answer- A genetic or a carrier test - Cystic fibrosis is a hereditary disease that im...

PHARMACOLOGY FINAL EXAM TESTBANK- CORRECT AND VERIFIED GUIDE 1. Both men and women experience bone loss with aging. The bones most likely to demonstrate significant loss are: a. Cortical bones b. Femoral neck bones c. Cervical vertebrae d. Pelvic bones 2. Bisphosphonates treat or prevent osteoporosis by: a. Inhibiting osteoclastic activity b. Fostering bone resorption c. Enhancing calcium uptake in the bone d. Strengthening the osteoclastic proton pump 3. Prophylactic use of bisphospho...