Western blotting - Study guides, Revision notes & Summaries

MCB 2050 Midterm W21 – Dr. Yankulov via Respondus Lockdown Browser and Webcam PART A – Questions from tutorial Question 1. You are studying the genes involved in embryonal development. Which of the following techniques could be used to visualize the locations with high gene expression of a particular gene in a whole mounted embryo? a) Western blotting b) Northern blotting c) Southern blotting d) Immunoprecipitation e) RNA in situ hybridization Question 2. Why is Taq DNA polymerase ...

MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph PART A 1. Which of the following is NOT an essential component of a cloning vector? a. A unique restriction endonuclease cleavage b. A dominant selectable marker gene c. An origin of replication d. A Taq polymerase binding site 2. Methylation of DNA sequence is used how by bacteria? a. Methylation of DNA can inhibit cell division b. Methyla...

Gel Electrophoresis Questions and Answers Rated A+ What role does gel electrophoresis play in gene therapy research? Gel electrophoresis is used to analyze the delivery and integration of therapeutic genes into target cells, helping assess the effectiveness of gene therapy approaches. How can gel electrophoresis help identify pathogens in a clinical setting? By amplifying specific genetic markers of pathogens and separating them using gel electrophoresis, researchers can quickly ide...

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

BCHM102 Midterm Exam Practice Questions and Answers aminoacyl tRNA synthetase requires ______ energy - Correct Answer️️ -ATP translocation requires ________ energy - Correct Answer️️ -GTP two methods of translation termination - Correct Answer️️ -hairpin formation and rho protein post-transcriptional modification - Correct Answer️️ -the modifications that occur to an mRNA transcript before it is exported from the nucleus as mature mRNA. Include: splicing of introns, addit...

Bio 205 Practice Guide Assessment Questions with Best Graded Marking Scheme 2024 Why is a nitrocellulose membrane used in western blotting? - correct answer stronger and can be used for other processes How do proteins move from the polyacrylamide gel onto nitrocellulose? - correct answer apply negative electrodes on gel side and positive on membrane side and flow contents from negative to positive What is the third stage of western blotting? - correct answer probing and hybridization W...

MCAT - Lab Techniques (MilesDown)Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass, t...

MCAT - Lab Techniques (MilesDown) Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass,...

Tools used in biotechnology and genetic engineering - Answer-In Silico analysis, DNA microarray analysis, gel electrophoresis, PCR, and RFLP analysis. PCR - Answer-*Polymerase Chain Reaction* technique for quickly and easily making many copies of even a very small amount of DNA Reverse Transcriptase - Answer-enzyme that turns single-stranded RNA into double-stranded DNA Complementary DNA (cDNA) - Answer-DNA produced from an RNA template using reverse transcriptase. often used to clone ...

DAT Mnemonics Practice Exam Questions & Answers. Cell Cycle - CORRECT ANSWER Go Sam Go Make Cake G1 growth phase 1 of interphase S synthesis of interphase G2 growth phase 2 of interphase M mitosis of M phase C cytokinesis of M phase Nitrogenous bases - CORRECT ANSWER PUR As Gold = purines = adenine and guanine CUT the PY = pyrimidines = cytosine, uracil, thymine Types of blots - CORRECT ANSWER SNOW DROP southern blotting = DNA northern blotting = RNA western blotting ...