Biochemistry Lecture and Exam Notes for Imperial College Students
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Protein Science Notes (Mass spectrometry & Glycolysation)
Second year student studying for a Biochemistry/Biotechnology degree 
 
Content: 
- Protein dynamics by NMR 
- Mass Spectrometry in great detail (includes ionisation sources, schematic diagrams, types of Mass spec like Quadrupole mass filters, etc.) 
- Parts of a mass spectrometer (Mass analyser, etc.) 
- Performance characteristics of each type of mass spectrometry 
- Types of ion detectors in its details 
- How to read Mass spec data (molecular ions/ fragmented ions and y ion/ b ion pathway)...
- Lecture notes
- • 40 pages •
Second year student studying for a Biochemistry/Biotechnology degree 
 
Content: 
- Protein dynamics by NMR 
- Mass Spectrometry in great detail (includes ionisation sources, schematic diagrams, types of Mass spec like Quadrupole mass filters, etc.) 
- Parts of a mass spectrometer (Mass analyser, etc.) 
- Performance characteristics of each type of mass spectrometry 
- Types of ion detectors in its details 
- How to read Mass spec data (molecular ions/ fragmented ions and y ion/ b ion pathway)...
Bioinformatics Lecture 1 Notes
Bioinformatics Lecture 1 Notes 
Second year student studying for a Biochemistry/Biotechnology degree 
 
Content: 
- Introduction to the course 
- Annotating sequences 
- Global/ Pairwise alignments 
- BLAST output 
- Substitution/ PAM/ BLOSUM matrix 
- Log odds 
- Deriving Matrix scores 
- Simplifying scores
- Package deal
- Lecture notes
- • 6 pages •
Bioinformatics Lecture 1 Notes 
Second year student studying for a Biochemistry/Biotechnology degree 
 
Content: 
- Introduction to the course 
- Annotating sequences 
- Global/ Pairwise alignments 
- BLAST output 
- Substitution/ PAM/ BLOSUM matrix 
- Log odds 
- Deriving Matrix scores 
- Simplifying scores
Bioinformatics Lecture 2 Notes
Bioinformatics Lecture 2 Notes 
Second year student studying for a Biochemistry/Biotechnology degree 
 
Content 
- Focussed Scoring Methods 
- Position-Specific Scoring Matrices (PSSM) including examples of matrices & using PSSM with PSI-BLAST 
- Pseudocounts 
- Protein domains and Functional sites 
- MSA ClustalO Output 
- PROSITE
- Package deal
- Lecture notes
- • 5 pages •
Bioinformatics Lecture 2 Notes 
Second year student studying for a Biochemistry/Biotechnology degree 
 
Content 
- Focussed Scoring Methods 
- Position-Specific Scoring Matrices (PSSM) including examples of matrices & using PSSM with PSI-BLAST 
- Pseudocounts 
- Protein domains and Functional sites 
- MSA ClustalO Output 
- PROSITE
Bioinformatics Lecture 4 Notes
Bioinformatics Lecture 4 Notes 
Second year student studying for a Biochemistry/Biotechnology degree 
 
Content 
- Fold prediction 
- Prediction by Homology 
- Phyre 
- Phylogenetic trees 
- Tree building programs: Distance based method, Bootstrapping, Maximum Parsimony.
- Package deal
- Lecture notes
- • 1 pages •
Bioinformatics Lecture 4 Notes 
Second year student studying for a Biochemistry/Biotechnology degree 
 
Content 
- Fold prediction 
- Prediction by Homology 
- Phyre 
- Phylogenetic trees 
- Tree building programs: Distance based method, Bootstrapping, Maximum Parsimony.
Bioinformatics Lecture 3 Notes
Bioinformatics module Lecture 3 
For a Second year student studying a Biochemistry/Biotechnology degree 
 
Content 
- Hidden Markov Models – HMM 
- Protein Structure Prediction 
- Intro to Secondary Structure Prediction 
- Jpred Algorithm 
- 2 types of networks (Sequence to structure, Structure to structure)
- Package deal
- Lecture notes
- • 1 pages •
Bioinformatics module Lecture 3 
For a Second year student studying a Biochemistry/Biotechnology degree 
 
Content 
- Hidden Markov Models – HMM 
- Protein Structure Prediction 
- Intro to Secondary Structure Prediction 
- Jpred Algorithm 
- 2 types of networks (Sequence to structure, Structure to structure)
Bioinformatics Lecture 4 Notes
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Bioinformatics Lecture 3 Notes