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Titration Curves of enzymes
How to make the titration curve of glycine and process data regarding this. Also showing principal concepts of: pH, PKa
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- • 8 pages •
How to make the titration curve of glycine and process data regarding this. Also showing principal concepts of: pH, PKa
The use of buffers answered worksheet
Understanding the use of buffers in Biological sciences, answered worksheet (pH...etc)
- Other
- • 10 pages •
Understanding the use of buffers in Biological sciences, answered worksheet (pH...etc)
Purifying proteins by ion exchange chromatography answered worksheet
Purifying proteins by ion exchange chromatography Aims of the experiment • To separate two proteins using difference in net charge. Your learning objectives • To understand the practical and theoretical basis of ion exchange chromatography through its application to protein separation. • To understand how columns are set up and run. • To be able to use the knowledge to devise separation procedures using alternative ion exchange resins. • To appr...
- Other
- • 14 pages •
Purifying proteins by ion exchange chromatography Aims of the experiment • To separate two proteins using difference in net charge. Your learning objectives • To understand the practical and theoretical basis of ion exchange chromatography through its application to protein separation. • To understand how columns are set up and run. • To be able to use the knowledge to devise separation procedures using alternative ion exchange resins. • To appr...
Affinity Chromatography answered Worksheet
To purify α-lactalbumin from milk whey, using affinity chromatography. To identify the proteins in cow’s milk whey To determine the purity of α-lactalbumin separated from milk whey
- Other
- • 17 pages •
To purify α-lactalbumin from milk whey, using affinity chromatography. To identify the proteins in cow’s milk whey To determine the purity of α-lactalbumin separated from milk whey
Thermodynamics of trypsin denaturation
To determine the activation energy for an enzyme catalysed reaction • To determine whether the thermal denaturation of trypsin is entropy or enthalpy driven
- Other
- • 17 pages •
To determine the activation energy for an enzyme catalysed reaction • To determine whether the thermal denaturation of trypsin is entropy or enthalpy driven
Answered Worksheet on Fluorescence assay
MEASUREMENT OF SERUM CHOLESTEROL USING A FLUORESCENCE ASSAY
- Other
- • 15 pages •
MEASUREMENT OF SERUM CHOLESTEROL USING A FLUORESCENCE ASSAY
Molecular Exclusion Chromatography of Haemoglobin
Worksheets + Answers of Experiments performed during Year 1 Biochemistry. To separate molecules on the basis of size difference. Your learning objectives · To understand the practical and theoretical basis of molecular exclusion chromatography. · To understand how columns are set up and run. · To understand the terms 'bed volume' and 'void volume' and how they are measured. · To be able to apply simple tests to identify the eluted proteins. · To appreciate some applicatio...
- Other
- • 23 pages •
Worksheets + Answers of Experiments performed during Year 1 Biochemistry. To separate molecules on the basis of size difference. Your learning objectives · To understand the practical and theoretical basis of molecular exclusion chromatography. · To understand how columns are set up and run. · To understand the terms 'bed volume' and 'void volume' and how they are measured. · To be able to apply simple tests to identify the eluted proteins. · To appreciate some applicatio...