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IB Biology HL topic 7

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IB biology notes for higher level topic 7

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☒•¥÷ ¥:☒.• E.÷I• ¥↳• • Nucleic Acids By A rathi Anand




0488




TO PICS 7 I 7 2,7 3
.
, . .

,DNA is 2M long

topic 7
Essential Idea : The structure of DNA is ideally suited to its function .




Skill :
Analysis of results of the
Hershey and chase experiment providing
evidence that DNA is the genetic material .




In the mid twentieth century , scientists were still unsure as to whether
-




DNA or protein was the genetic material of the cell .





Known that some viruses consisted solely of DNA and a protein coat
and could transfer their genetic material into hosts .


→ 1952 , Alfred and Martha Chase conducted
Hershey a series of
experiments to prove that DNA was the
genetic material .




viruses ( T2 bacteriophage) were grown in one of two
isotopic
mediums in order to radioactively
viral component
label a
specific .




viruses grown in radioactive sulfur ( 3 s s) had radiolabelled
proteins



( sulfur is present in proteins but not DNA ) 32 P )
.




viruses grown in radioactive phosphorus ( had radiolabeled
DNA (
phosphorus is present in DNA but not proteins ) .




The viruses were then allowed to infect a bacterium (E -
coli ) and the
the virus and bacteria were separated via
centrifugation .



• The larger bacteria formed a solid pallet while the smaller
viruses remained in the supernatant .



bacterial pallet was found to be radioactive when infected
The
32
by
the p viruses ( DNA ) but not the 35 S
-
viruses ( proteins ) -
.


• This demonstrated that DNA , NOT protein , was the genetic
material because DNA was transferred to the bacteria .




- -




protein part not in

of phage phage Ghosts




Application Rosalind Franklin 's
: and Maurice Wilkins ' investigation of DN

ray diffraction
structure by X -
.




Rosalind Franklin and Maurice Wilkins used a method of X -

ray
diffraction to investigate the structure of DNA .

, DNA was
purified and then fibres were stretched thin glass tube

in a

(to make most of the strands parallel )

The DNA was targeted by a X -

ray beam , which was diffracted
when it contacted an atom .




The scattering pattern ray was recorded
of the X film and
• -
on a

used to elucidate details of molecular structure .




Summary of the Process of X-Ray Crystallography
From the
scattering pattern
produced by a DNA molecule ,

certain inferences could be
made about its structure .





COMPOSITION :
DNA is a

double stranded molecule
• ORIENTATION : Nitrogenous
bases are closely packed together on the inside and phosphates
form an outer backbone .




• SHAPE ! The DNA molecule twists at regular intervals (every 34
Ang storm ) to form a helix ( two strands = double helix .




Franklin 's data shared by Wilkins with James Watson ( without
was
Franklin 's permission ) who , with the help of Francis Crick , used
the information to create a molecular model of the basic structu
of DNA .


→ In 1962 , Watson , Crick and Wilkins ( but not Franklin ) were awarde
Nobel prize .




understanding : DNA structure
suggested a mechanism for DNA
replication .




FrankKin 's X ray diffraction experiments demonstrated that the DNA
-




helix is both tightly packed and regular in structure .



Phosphates ( and sugars ) form the outer backbone and nitrogenous
within the interior
bases are packaged .




Chargaff demonstrated that DNA is composed of an equal numbe
of purines ( At a) and (CtT) pyrimidines .

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